PXD002871

PXD002871 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics quantitative characterization in Jurkat E6.1 and Jurkat Gamma1.
Description	Phosphorylation is a reversible post-translational modification, playing a vital role in protein function. In T cells, protein phosphorylation is the key mechanism regulating T cell receptor – driven signaling pathways. In order to gain insights into the phosphoproteome evolution of T cell activation, we performed a large-scale quantitative phosphoproteomics study of Jurkat E6.1 (wild type) and Jurkat gamma1 (Phospholipase gamma1 null) cell clones upon costimulation with anti-CD3 and anti-CD28 antibodies at times ranging from 15 min to as long as 120 min. In total, we identified 5585 phosphopeptides belonging to 2008 phosphoproteins from both cell clones. We detected 130 and 114 novel phosphopeptides in Jurkat E6.1 and Jurkat gamma1 clones, respectively. A significantly lower number of proteins containing regulated phosphorylation sites were identified in Jurkat gamma1 in comparison to Jurkat E6.1, reflecting the vital role of Phospholipase gamma1 in T cell signaling. Several new phosphorylation sites from lymphocyte-specific protein tyrosine kinase (LCK) were identified. Of these, Serine-121 showed significant changes in JE6.1 while only small changes in the Jgamma1 clone.
HostingRepository	PRIDE
AnnounceDate	2015-11-10
AnnouncementXML	Submission_2015-11-10_03:28:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Montserrat Carrascal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-07 08:35:48	ID requested
⏵ 1	2015-11-10 03:28:45	announced

Nguyen TD, Carrascal M, Vidal-Cortes O, Gallardo O, Casas V, Gay M, Phan VC, Abian J, The phosphoproteome of human Jurkat T cell clones upon costimulation with anti-CD3/anti-CD28 antibodies. J Proteomics, 131():190-198(2016) [pubmed]

ProteomeXchange project tag: Human Proteome Project

curator keyword: Biological

submitter keyword: Phosphorylation, Jurkat T cells, CD3/CD28 costimulation, phospholipase C gamma 1, quantitative phosphoproteomics.

Joaquin Abian Moñux
contact affiliation	CSIC/UAB Proteomics Laboratory, IIBB-CSIC, Barcelona, Spain
contact email	joaquim.abian.csic@uab.cat
lab head
Montserrat Carrascal
contact affiliation	IIBB-CSIC
contact email	montserrat.carrascal.csic@uab.cat
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD002871

PRIDE project URI

• PRIDE
  1. PXD002871
     1. Label: PRIDE project
     2. Name: Phosphoproteomics quantitative characterization in Jurkat E6.1 and Jurkat Gamma1.