PXD002866 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The Physcomitrella patens chloroplast proteome changes in response to short-term stress |
Description | Plants often face more than one type of stress at a time, and how plants respond to combined stress factors is therefore of great interest. Here, we used protoplasts of the moss Physcomitrella patens as a model to study the effects of short-term combined stress on the chloroplast proteome. In this system, biotic stress is mimicked by exposure to cell wall-hydrolyzing enzymes, and abiotic stress is related to plasmolysis. We used SWATH-MS to perform label-free comparative quantitative proteomic analysis of the chloroplast proteome. Overall, we quantified 480 chloroplast proteins, 220 of which showed a more than 1.4-fold change in abundance in protoplasts. We additionally quantified 1422 chloroplast proteins using emPAI. We observed degradation of a significant portion of the chloroplast proteome during the first hour of stress. Electron-transport chain (ETC) components underwent the heaviest degradation, resulting in the decline of photosynthetic activity. We also compared the proteome changes to those in the transcriptional level of nuclear-encoded chloroplast genes. Globally, the levels of the quantified proteins and their corresponding mRNAs showed limited correlation. Genes involved in the biosynthesis of chlorophyll and components of the outer chloroplast membrane showed decreases in both transcript and protein abundance. However, proteins like dehydroascorbate reductase 1 and 2-cys peroxiredoxin B responsible for ROS detoxification increased in abundance. Further, genes such as thylakoid ascorbate peroxidase were induced at the transcriptional level but down-regulated at the proteomic level. Together, our results demonstrate that the initial chloroplast reaction to stress is due changes at the proteomic level. |
HostingRepository | PRIDE |
AnnounceDate | 2017-10-24 |
AnnouncementXML | Submission_2017-10-24_03:29:19.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD002866 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Georgij Arapidi |
SpeciesList | scientific name: Physcomitrella patens subsp. patens (Moss); NCBI TaxID: 3218; |
ModificationList | Ammonia-loss; Dehydrated; Acetyl; Carbamidomethyl |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-09-07 03:27:54 | ID requested | |
1 | 2017-01-03 03:01:40 | announced | |
⏵ 2 | 2017-10-24 03:29:21 | announced | Updated project metadata. |
Publication List
Fesenko I, Seredina A, Arapidi G, Ptushenko V, Urban A, Butenko I, Kovalchuk S, Babalyan K, Knyazev A, Khazigaleeva R, Pushkova E, Anikanov N, Ivanov V, Govorun VM, Chloroplast Proteome Changes in Response to Protoplastation. Front Plant Sci, 7():1661(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: SWATH-MS, chloroplast proteome, moss Physcomitrella patens, label-free quantification, stress |
Contact List
Georgij Arapidi |
contact affiliation | Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences |
contact email | arapidi@gmail.com |
lab head | |
Georgij Arapidi |
contact affiliation | IBCH RAS |
contact email | arapidi@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002866
- Label: PRIDE project
- Name: The Physcomitrella patens chloroplast proteome changes in response to short-term stress