PXD002860

PXD002860 is an original dataset announced via ProteomeXchange.

Title	MCF7 SILAC Comparison of Protein Expression for Acidosis Biomarkers
Description	MCF7 cells were grown in culture under low pH and physiological pH conditions in order to generate candidate biomarkers for tumor acidosis. SILAC was used to enable comparison of protein expression levels using relative quantification. Two biological replicates were prepared using "label flipping," and each fraction was analyzed in duplicate by LC-MS/MS. Sample nomenclature includes N for "normal" or physiological pH versus L for low pH as the first letter with H for "heavy" and L for "light" as the second letter. Therefore, one experiment is described as NH-LL, while the other is NL-LH. Fifteen IEF fractions were generated and analyzed with LC-MS/MS (RSLC and Orbitrap, Thermo) for each SILAC pair .
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:08:33.059.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Koomen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled L-lysine; 6x(13)C; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-07 01:53:31	ID requested
1	2021-03-04 11:31:24	announced
⏵ 2	2024-10-22 05:08:39	announced	2024-10-22: Updated project metadata.

Damaghi M, Tafreshi NK, Lloyd MC, Sprung R, Estrella V, Wojtkowiak JW, Morse DL, Koomen JM, Bui MM, Gatenby RA, Gillies RJ, Chronic acidosis in the tumour microenvironment selects for overexpression of LAMP2 in the plasma membrane. Nat Commun, 6():8752(2015) [pubmed]

10.1038/ncomms9752;

curator keyword: Biomedical

submitter keyword: Breast Cancer, SILAC,MCF7 Cells, Acidosis

Robert Gillies
contact affiliation	Cancer Imaging and Metabolism Moffitt Cancer Center Tampa, FL USA
contact email	robert.gillies@moffitt.org
lab head
John Koomen
contact affiliation	Moffitt Cancer Center
contact email	john.koomen@moffitt.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD002860

PRIDE project URI

• PRIDE
  1. PXD002860
     1. Label: PRIDE project
     2. Name: MCF7 SILAC Comparison of Protein Expression for Acidosis Biomarkers