PXD002858

PXD002858 is an original dataset announced via ProteomeXchange.

Title	Mouse purified hepatocyte secretome iTRAQ LC/MS
Description	This study tests whether hepatic steatosis, independent of inflammation, alters the hepatocyte protein secretory profile, and examines whether changes in the secretory products contribute to the development of metabolic dysfunction in other cell types. Mouse hepatocytes were isolated by flow cytometry and cultured in serum-free conditions. Conditioned media was used for LC/MS analysis to compare hepatocytes from chow fed animals to high-fat diet animals with liver steatosis.
HostingRepository	PRIDE
AnnounceDate	2018-03-14
AnnouncementXML	Submission_2018-03-14_05:40:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christoph Krisp
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-07 01:42:09	ID requested
⏵ 1	2018-03-14 05:40:37	announced

Meex RC, Hoy AJ, Morris A, Brown RD, Lo JC, Burke M, Goode RJ, Kingwell BA, Kraakman MJ, Febbraio MA, Greve JW, Rensen SS, Molloy MP, Lancaster GI, Bruce CR, Watt MJ, Fetuin B Is a Secreted Hepatocyte Factor Linking Steatosis to Impaired Glucose Metabolism. Cell Metab, 22(6):1078-89(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: mouse, secreted, liver, high-fat diet

Matthew Watt
contact affiliation	Department of Physiology Monash University VIC, 3800, Australia
contact email	matthew.watt@monash.edu
lab head
Christoph Krisp
contact affiliation	Australian Proteome Analysis Facility (APAF)
contact email	ckrisp@proteome.org.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD002858

PRIDE project URI

• PRIDE
  1. PXD002858
     1. Label: PRIDE project
     2. Name: Mouse purified hepatocyte secretome iTRAQ LC/MS