PXD002855

PXD002855 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A novel inducible retroviral expression system for StrepHA-tandem affinity purification mass-spectrometry-based proteomics
Description	Tandem-affinity purification mass spectrometry using the streptavidin-hemagglutinin (SH)-tag has been successfully employed to map signaling networks in several large-scale studies. However its application has so far been restricted to the small number of Flp/FRT-recombination competent cell lines. We present pRSHIC, a novel retroviral, doxycycline-inducible Tet-On vector system suitable for expression of SH-tagged target proteins in a wide range of human and mouse cell systems. The additional feature of concomitant reporter fluorophore expression makes pRSHIC a valuable tool for a diverse set of phenotypic analyses beyond TAP-MS experiments. The dataset demonstrates the application of pRSHIC for TAP-MS analysis of two showcase bait proteins involved in cancer cell proliferation as well as cell death induction and identified novel high- confidence interacting proteins with possible pharmacological intervention potential.
HostingRepository	PRIDE
AnnounceDate	2016-01-05
AnnouncementXML	Submission_2016-01-05_15:12:08.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002855
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Richard Kumaran Kandasamy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-07 00:00:01	ID requested
⏵ 1	2016-01-05 15:12:09	announced

Publication List

Keyword List

submitter keyword: protein-protein interaction, tandem affinity purification-mass spectrometry, streptavidin-hemagglutinin-tag

submitter keyword: protein-protein interaction, tandem affinity purification-mass spectrometry, streptavidin-hemagglutinin-tag

Contact List

Giulio Superti-Furga
contact affiliation	Scientific Director, CeMM - Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria; Professor for Medical Systems Biology, Center for Physiology and Pharmacology, Medical University of Vienna, Vienna, Austria (lab head)
contact email	GSuperti@cemm.oeaw.ac.at
lab head
Richard Kumaran Kandasamy
contact affiliation	CeMM Center for Molecular Medicine
contact email	rkandasamy@cemm.oeaw.ac.at
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002855

PRIDE project URI

Repository Record List

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