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PXD002851

PXD002851 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomics reveals an important role of GsCBRLK in salt stress response of soybean
DescriptionSalinity is one of the serious environmental constraints stresses limiting crop plants growth and yield. We have previously reported that GsCBRLK functions as a positive regulator of plant tolerance to salt stress. In order to investigate the physiological and molecular mechanisms underlying the salinity tolerance regulated by GsCBRLK, the gene was overexpressed in soybean plants. Here we examined the salt-responsive proteomes of the GsCBRLK overexpression soybean and wild typeWT plants using iTRAQ-based proteomic approach to elucidate investigate the global effects and potentialssible downstream targets of GsCBRLK protein. A total of 941 proteins showed significant changes in protein abundance in soybean leaves, and 574 of the NaCl-regulated proteins were GsCBRLK-dependent. Among the identified proteins, four protein changes in both the two genotypes after NaCl treatment were validated using Western blot analysis. The iIdentification of the salt-reponsive proteins has revealed the involvement of GsCBRLK protein in the enhancement of ROS scavenging and photosynthesis capacity in soybean plants, which was corrobarated with the physiological effects of GsCBRLK overexpression. More importantly, the proteomic data has suggested the regulatory function of GsCBRLK in salt signal transduction pathway mediated by Ca2+/CaM. These findings have contributed to our knowledge of plant GsCBRLK mediated salt tolerance mechanisms mediated by GsCBRLK.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:25:13.174.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWei Ji
SpeciesList scientific name: Glycine max; NCBI TaxID: 3847;
ModificationListiTRAQ8plex-116 reporter+balance reagent acylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-09-04 04:31:34ID requested
12016-05-23 01:03:08announced
22024-10-22 04:25:15announced2024-10-22: Updated project metadata.
Publication List
10.3389/fpls.2016.00573;
Ji W, Cong R, Li S, Li R, Qin Z, Li Y, Zhou X, Chen S, Li J, Comparative Proteomic Analysis of Soybean Leaves and Roots by iTRAQ Provides Insights into Response Mechanisms to Short-Term Salt Stress. Front Plant Sci, 7():573(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: soybean, salt stress, GsCBRLK
Contact List
Wei Ji
contact affiliationNortheast Agricultural University, St. Mucai, Harbin, Heilongjiang, 150030, China
contact emailiwei_ji@neau.edu.cn
lab head
Wei Ji
contact affiliationNortheast Agricultural University
contact emailiwei_ji@neau.edu.cn
dataset submitter
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Dataset FTP location
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