<<< Full experiment listing

PXD002850

PXD002850 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleROS-activated ATM-dependent phospho-signaling
DescriptionATM (ataxia-telangiectasia mutated) protein plays a central role in phosphorylating a network of proteins in response to DNA double strand breaks. These phosphorylated proteins function in signalling pathways designed to maintain the stability of the genome and minimize the risk of disease by controlling cell cycle checkpoints, initiating DNA repair and regulating gene expression. We employed a modified TiSH global quantitative phosphoproteomics approach to identify cytoplasmic proteins altered in their phosphorylation state in control and A-T cells in response to oxidative damage. We demonstrated that ATM was activated by oxidative damage in the cytoplasm as well as in the nucleus and identified a total of 9,612 phosphorylation sites including 6,650 high confidence sites mapping to 2,536 unique proteins.
HostingRepositoryPRIDE
AnnounceDate2016-01-04
AnnouncementXMLSubmission_2016-01-04_14:24:56.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMark Graham
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-09-04 02:51:28ID requested
12016-01-04 14:24:58announced
Publication List
Kozlov SV, Waardenberg AJ, Engholm-Keller K, Arthur JW, Graham ME, Lavin M, Reactive Oxygen Species (ROS)-Activated ATM-Dependent Phosphorylation of Cytoplasmic Substrates Identified by Large-Scale Phosphoproteomics Screen. Mol Cell Proteomics, 15(3):1032-47(2016) [pubmed]
Keyword List
curator keyword: Biomedical, Biological
submitter keyword: ATM, phosphoproteomics, reactive oxygen species, fibroblast
Contact List
Mark Graham
contact affiliationChildren's Medical Research Institute
contact emailmgraham@cmri.org.au
lab head
Mark Graham
contact affiliationCell Signalling
contact emailmgraham@cmri.org.au
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002850
PRIDE project URI
Repository Record List
[ + ]