PXD002850 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | ROS-activated ATM-dependent phospho-signaling |
Description | ATM (ataxia-telangiectasia mutated) protein plays a central role in phosphorylating a network of proteins in response to DNA double strand breaks. These phosphorylated proteins function in signalling pathways designed to maintain the stability of the genome and minimize the risk of disease by controlling cell cycle checkpoints, initiating DNA repair and regulating gene expression. We employed a modified TiSH global quantitative phosphoproteomics approach to identify cytoplasmic proteins altered in their phosphorylation state in control and A-T cells in response to oxidative damage. We demonstrated that ATM was activated by oxidative damage in the cytoplasm as well as in the nucleus and identified a total of 9,612 phosphorylation sites including 6,650 high confidence sites mapping to 2,536 unique proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2016-01-04 |
AnnouncementXML | Submission_2016-01-04_14:24:56.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mark Graham |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-09-04 02:51:28 | ID requested | |
⏵ 1 | 2016-01-04 14:24:58 | announced | |
Publication List
Kozlov SV, Waardenberg AJ, Engholm-Keller K, Arthur JW, Graham ME, Lavin M, Reactive Oxygen Species (ROS)-Activated ATM-Dependent Phosphorylation of Cytoplasmic Substrates Identified by Large-Scale Phosphoproteomics Screen. Mol Cell Proteomics, 15(3):1032-47(2016) [pubmed] |
Keyword List
curator keyword: Biomedical, Biological |
submitter keyword: ATM, phosphoproteomics, reactive oxygen species, fibroblast |
Contact List
Mark Graham |
contact affiliation | Children's Medical Research Institute |
contact email | mgraham@cmri.org.au |
lab head | |
Mark Graham |
contact affiliation | Cell Signalling |
contact email | mgraham@cmri.org.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002850 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002850
- Label: PRIDE project
- Name: ROS-activated ATM-dependent phospho-signaling