PXD002845

PXD002845 is an original dataset announced via ProteomeXchange.

Title	Deep quantitative proteomics underlies extensive metabolic reprogramming and cancer-like changes of ectopic endometriotic stromal cells
Description	Endometriosis is a prevalent health condition in women of reproductive age characterized by ectopic growth of endometrial tissue in the extrauterine environment. Thorough understanding of the molecular mechanisms underlying the disease are still lacking and incomplete. We dissect eutopic and ectopic endometrial primary stromal cell proteomes to a depth of nearly 6900 proteins using quantitative mass-spectrometry with a spike-in SILAC standard. Acquired data reveal metabolic reprogramming of ectopic stromal cells of endometriosis with extensive upregulation of glycolysis and down-regulation of oxidative respiration – a wide-spread metabolic phenotype previously described in many cancers. Our results also underlie other molecular changes of ectopic endometriotic stromal cells indicating reduced apoptotic potential, increased cellular adhesiveness/invasiveness and altered immune function. The changes related to metabolism are additionally reflected by attenuated aerobic respiration of ectopic endometrial stromal cells measured by live cell oximetry and by altered mRNA levels. These comprehensive proteomics data refine the current understanding of endometriosis presenting potential new avenues for therapies.
HostingRepository	PRIDE
AnnounceDate	2015-12-21
AnnouncementXML	Submission_2015-12-21_05:43:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sergo Kasvandik
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deamidated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-03 00:24:26	ID requested
⏵ 1	2015-12-21 05:43:55	announced

Kasvandik S, Samuel K, Peters M, Eimre M, Peet N, Roost AM, Padrik L, Paju K, Peil L, Salumets A, Deep Quantitative Proteomics Reveals Extensive Metabolic Reprogramming and Cancer-Like Changes of Ectopic Endometriotic Stromal Cells. J Proteome Res, 15(2):572-84(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Endometriosis, Warburg effect, endometrium, stromal cell, proteomics, SILAC

Andres Salumets
contact affiliation	1. Competence Centre on Health Technologies, Tiigi 61b, Tartu, Estonia 2. Chair of Obstetrics and Gynaecology, Institute of Biomedicine, University of Tartu, Ravila 19, Tartu, Estonia
contact email	andres.salumets@ccrmb.ee
lab head
Sergo Kasvandik
contact affiliation	University of Tartu, Proteomics core facility
contact email	sergo.kasvandik@ut.ee
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/12/PXD002845

PRIDE project URI

• PRIDE
  1. PXD002845
     1. Label: PRIDE project
     2. Name: Deep quantitative proteomics underlies extensive metabolic reprogramming and cancer-like changes of ectopic endometriotic stromal cells