PXD002842

PXD002842 is an original dataset announced via ProteomeXchange.

Title	A Mass Spectrometry Approach Reveals a New Toxic Role For Alzheimer’s Disease Aβ Peptide: SPLICEOSOME IMPAIRMENT
Description	Alzheimer’s disease is the most common form of dementia, and its prevalence increases exponentially with age. The patients affected gradually lose cognitive function, control over their sense of orientation, their emotions, and other aspects of behavior. Thirty-five million people are now considered to be affected by AD and this number is expected to double in the next few decades. We utilized an AD cell model system for a proteomic study by using mass spectrometry. To mimic early events in AD, LAN5 neuroblastoma cell were incubated for a short time with a recombinant form of Aβ42 (rAβ42), a peptide involved in AD, and the extracted proteins were utilized for the proteome analysis. Furthermore, we used bioinformatics tools to identify networks, associated processes, pathways, etc. The potential modulation of pathways suggested by the similarity of GO terms and presence of protein-protein interaction networks among significantly modulated proteins was explored using the bioinformatic tool KEGG. Pathway enrichment analysis was conducted for up and down regulated protein groups, and four pathways were identified. In particular, the Spliceosome pathway identified in the under-expressed protein group was reported by KEGG to be the most significantly enriched. To confirm the effect of Aβ42 on the spliceosomal pathway, the level of expression of SmB/B’/N (a component of the spliceosomal machinery) was measured. However, further studies are necessary to fully elucidate the the down-regulation effect of the spliceosome proteins in AD, and how this may contribute to the early event in this disorder.
HostingRepository	PRIDE
AnnounceDate	2017-02-06
AnnouncementXML	Submission_2017-02-06_01:05:46.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002842
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Domenico Nuzzo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-02 07:11:02	ID requested
⏵ 1	2017-02-06 01:05:48	announced

Nuzzo D, Inguglia L, Walters J, Picone P, Di Carlo M, Peptide: Spliceosome Impairment. J Proteome Res, 16(4):1526-1541(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Alzheimer’s disease, Aβ42, splicing, AD early events, orbitrap, Human

Marta Di Carlo
contact affiliation	Istituto di Biomedicina ed Immunologia Molecolare (IBIM) CNR, Via U. La Malfa 153, Palermo 90146, Italy
contact email	marta.dicarlo@ibim.cnr.it
lab head
Domenico Nuzzo
contact affiliation	National Research Council of Italy (CNR), Institute of Biomedicine and Molecular Immunology (IBIM), Palermo, Italy
contact email	domenico.nuzzo@cnr.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002842
     1. Label: PRIDE project
     2. Name: A Mass Spectrometry Approach Reveals a New Toxic Role For Alzheimer’s Disease Aβ Peptide: SPLICEOSOME IMPAIRMENT