PXD002828

PXD002828 is an original dataset announced via ProteomeXchange.

Title	Anopheles culicifacies species A and species B Midgut Proteome
Description	Background: Mosquito midgut is an important target for the host parasite interaction studies as it plays a major role in parasite growth and maturation and vector susceptibility. Proteomic approaches coupled with bioinformatics analysis have been used to study expression of functional proteins/enzymes of An. culicifacies susceptible and refractory species midgut in order to understand the mechanism of refractoriness that may help in contributing to unravel the host pathogen interactions. Methodology/Principle findings: In the present study proteomics approaches namely in solution and in gel digestion strategies followed by LC/MS/MS analysis were used. Further bioinformatics analysis were carried out to find out the functional annotated proteins, biological process, molecular function and their sub-cellular location using Gene ontology, SMART analysis, CELLO etc. In solution and in gel approach coupled with LC-MS/MS identified a total of 91 proteins in susceptible species and 69 proteins in refractory species. Comparative analysis between susceptible and refractory species of An. culicifacies indicated that mainly proteins involved in proteolysis mechanism, catalytic activity, peptidases activity and immune related proteins were found to be dominating in refractory as compared to susceptible species. Conclusion/Significance: Based on the present data a significant increase in number of proteins in midgut of refractory An. culicifacies species B were found that may conclude that these proteins may be responsible for the inhibiting parasite growth and linked to the melanization of oocysts or parasite lysis mechanisms in natural populations of refractory mosquito. Hence the progress of our studies at protein level suggests that these identified annotated putative proteins/enzymes may help to explore natural vector-parasite systems and reveal valuable insights into the mechanism of refractoriness which in turn further useful for bringing of novel strategies for control of malaria.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:25:47.923.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	sonam vijay
SpeciesList	scientific name: Anopheles culicifacies A; NCBI TaxID: 63366; scientific name: Anopheles culicifacies B; NCBI TaxID: 63367;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Thermo Scientific instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2015-09-01 01:34:24	ID requested
1	2018-10-24 07:59:58	announced
⏵ 2	2024-10-22 04:25:51	announced	2024-10-22: Updated project metadata.

10.1186/s12864-018-4729-3;

Vijay S, Rawal R, Kadian K, Singh J, Adak T, Sharma A, Proteome-wide analysis of Anopheles culicifacies mosquito midgut: new insights into the mechanism of refractoriness. BMC Genomics, 19(1):337(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Anopheles, LC/MS/MS, Refractory, Midgut

Dr Arun Sharma
contact affiliation	Dr. Arun Sharma Protein Biochemistry and Structural Biology Division National Institute of Malaria Research (ICMR) Sector-8, Dwarka, New Delhi- 110077 E-mail: arushar@gmail.com
contact email	arushar@gmail.com
lab head
sonam vijay
contact affiliation	National Institute of Malaria Research
contact email	sonam.vijay@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002828
     1. Label: PRIDE project
     2. Name: Anopheles culicifacies species A and species B Midgut Proteome