PXD002821

PXD002821 is an original dataset announced via ProteomeXchange.

Title	Low mass blood peptides discriminative of IBD severity
Description	The enriched low mass proteome is unexplored as a source of differentiators for diagnosing and monitoring Inflammatory Bowel Disease (IBD) activity, less invasively than colonoscopy and histopathology. Differences in the enriched low mass plasma proteome (<25kDa) were assessed by label-free quantitative mass-spectrometry. A panel of marker candidates were progressed to validation phase and ‘Tier-2’ FDA-level validated quantitative assay. Proteins important in maintaining gut barrier function and homeostasis at the epithelial interface have been quantitated by Multiple Reaction Monitoring (MRM) in plasma and serum including both inflammatory rheumatoid arthritis controls (RA) and non-inflammatory healthy controls (C), ulcerative colitis (UC) and crohn’s disease (CD) patients. Detection by immunoblot confirmed presence at the protein level in serum. Correlation analysis and receiver operator characteristics were used to report the sensitivity and specificity. Five peptides discriminating IBD activity and severity had very little-to-no correlation to Erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), white cell or platelet counts. Three of these peptides were found to be binding partners to SPP24 protein alongside other known matrix proteins. These Proteins have the potentially improve effective diagnosis and evaluate IBD activity, reducing the need for more invasive techniques.
HostingRepository	PRIDE
AnnounceDate	2015-11-06
AnnouncementXML	Submission_2015-11-06_03:06:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Valerie Wasinger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acrylamidyl cysteinyl; phosphorylated residue
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-31 01:11:43	ID requested
⏵ 1	2015-11-06 03:06:46	announced

Yau YY, Duo X, Leong RW, Wasinger VC, Reverse-polynomial dilution calibration methodology extends lower limit of quantification and reduces relative residual error in targeted peptide measurements in blood plasma. Mol Cell Proteomics, 14(2):441-54(2015) [pubmed]

Wasinger VC, Yau Y, Duo X, Zeng M, Campbell B, Shin S, Luber R, Redmond D, Leong RW, Low Mass Blood Peptides Discriminative of Inflammatory Bowel Disease (IBD) Severity: A Quantitative Proteomic Perspective. Mol Cell Proteomics, 15(1):256-65(2016) [pubmed]

curator keyword: Biological

submitter keyword: Biomarker, IBD, proteomic, MRM, SPP24, GUC2A

Valerie Wasinger
contact affiliation	The University of NSW, Australia
contact email	v.wasinger@unsw.edu.au
lab head
Valerie Wasinger
contact affiliation	UNSW
contact email	v.wasinger@unsw.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD002821

PRIDE project URI

• PRIDE
  1. PXD002821
     1. Label: PRIDE project
     2. Name: Low mass blood peptides discriminative of IBD severity