PXD002815

PXD002815 is an original dataset announced via ProteomeXchange.

Title	A human interactome in three quantitative dimensions organized by stoichiometries and abundances
Description	The organization of a cell emerges from the interactions in protein networks. The interactome is critically dependent on the strengths of interactions and the cellular abundances of the connected proteins, both of which span orders of magnitude. However, these aspects have not yet been analyzed globally. Here, we have generated a library of HeLa cell lines expressing 1,125 GFP-tagged proteins under near-endogenous control, which we used as input for a next-generation interaction survey. Using quantitative proteomics, we detect specific interactions, estimate interaction stoichiometries and measures cellular abundances of interacting proteins. These three quantitative dimensions reveal that the protein network is dominated by weak, substoichiometric interactions that play a pivotal role in defining network topology. The minority of stable complexes can be identified by their unique stoichiometry signature. This study provides a rich interaction dataset connecting thousands of proteins, and introduces a framework for quantitative network analysis.
HostingRepository	PRIDE
AnnounceDate	2015-10-24
AnnouncementXML	Submission_2015-10-27_04:31:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-28 07:46:42	ID requested
1	2015-10-24 09:46:50	announced
⏵ 2	2015-10-27 04:31:14	announced	Updated publication reference for PubMed record(s): 26496610.

Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M, A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell, 163(3):712-23(2015) [pubmed]

curator keyword: Biological

submitter keyword: human interactome, protein interactions, stoichiometries, protein abundances

Matthias Mann
contact affiliation	Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry, Martinsried, Germany
contact email	mmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002815
     1. Label: PRIDE project
     2. Name: A human interactome in three quantitative dimensions organized by stoichiometries and abundances