PXD002810

PXD002810 is an original dataset announced via ProteomeXchange.

Title	miR-17-92 sustains lymphoma growth by fine-tuning MYC
Description	The synergism between c-MYC and miR-17-19b, a truncated version of the miR-17-92 cluster, is well documented during tumor initiation. However, little is known about miR-17-19b function in established cancers. Here we investigate the role of miR-17-19b in c-MYC-driven lymphomas by integrating SILAC-based quantitative proteomics, transcriptomics and 3’ UTR analysis upon miR-17-19b overexpression. We identify over one hundred novel miR-17-19b targets, of which 40% are co-regulated by c-MYC. Down-regulation of a new miR-17/20 target Chek2 increases the recruitment of HuR to c-MYC transcripts, resulting in the inhibition of c-MYC translation and thus interfering with in vivo tumor growth. Hence, in established lymphomas, miR-17-19b fine-tunes c-MYC activity through a tight control of its function and expression, ultimately ensuring cancer cell homeostasis. Our data highlight the plasticity of miRNA function, reflecting changes in the mRNA landscape and 3’ UTR shortening at different stages of tumorigenesis.
HostingRepository	PRIDE
AnnounceDate	2015-11-06
AnnouncementXML	Submission_2015-11-16_06:33:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Bremang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; N-acetylated residue
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-27 23:43:28	ID requested
1	2015-11-06 07:19:25	announced
⏵ 2	2015-11-16 06:33:26	announced	Updated publication reference for PubMed record(s): 26555894.

Mihailovich M, Bremang M, Spadotto V, Musiani D, Vitale E, Varano G, Zambelli F, Mancuso FM, Cairns DA, Pavesi G, Casola S, Bonaldi T, miR-17-92 fine-tunes MYC expression and function to ensure optimal B cell lymphoma growth. Nat Commun, 6():8725(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: miR-17-92, LC-MSMS, MYC, lymphoma, Chek2, microRNA

Tiziana Bonaldi
contact affiliation	Department of Experimental Oncology, European Institute of Oncology, Italy
contact email	tiziana.bonaldi@ieo.eu
lab head
Michael Bremang
contact affiliation	European Institute of Oncology
contact email	michael.bremang@ieo.eu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD002810

PRIDE project URI

• PRIDE
  1. PXD002810
     1. Label: PRIDE project
     2. Name: miR-17-92 sustains lymphoma growth by fine-tuning MYC