PXD002796

PXD002796 is an original dataset announced via ProteomeXchange.

Title	Quantitative Analysis of Differential Proteomes in Mouse Mesenchymal Stem Cells Converted to Salivary Epithelial Precursors
Description	Severe hyposalivation often results from Sjögren’s syndrome or radiation therapy for head and neck cancer. Devastating consequences of hyposalivation, such as rampant dental caries and persistent oral candidiasis, compromise the quality of life in those patients. Clinical management for dry mouth typically involves simple palliative methods or secretagogues, which are designed to stimulate saliva secretion from residual salivary acinar cells present in the glands. However, direct interventions in chronic dryness have yet to be employed in the clinical setting. Despite numerous studies on salivary gland regeneration, the molecular basis governing stem cell transdifferentiation into salivary epithelial precursors (SEP) is largely unknown. Our previously published study clearly indicated mouse bone marrow-derived mesenchymal stem cells (BM-MSCs) can be differentiated into SEP in vitro when co-cultured with isolated primary salivary gland cells without cell-to-cell contact. Our current study utilized iTRAQ-LS-MS/MS-based quantitative proteomics to profile key regulatory factors involved in mouse MSC-to-SEP conversion. We identified 280 differentially expressed proteins in BM-MSCs over the course of 7 days of co-culture. Interestingly, protein expression of salivary transcription factors (STFs), such as transcription factor E2a (TCF3), high mobility group protein 20B (HMG20b), and ankyrin repeat domain-containing protein 56 (ANKRD56) were increased in a time-dependent manner. Notably, pancreas specific transcription factor 1a (PTF1α), muscle, intestine and stomach expression-1 (MIST-1) and achaete-scute complex homolog 3(ASCL3) were newly induced over time in differentiated MSCs. We also identified the expression of Ptf1α in the mouse salivary glands for the first time and verified its expression in independent batches of co-cultured MSCs by western blotting. Furthermore, simulation of the molecular network involving the identified STFs has demonstrated evidence for their perspective roles in salivary gland development during glandular maturation. Thus, our study provides the first extensive proteomic profile of MSC-to-SEP transdifferentiation and identifies novel STFs that may be critical for this process.
HostingRepository	PRIDE
AnnounceDate	2017-02-14
AnnouncementXML	Submission_2017-02-14_05:24:12.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002796
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jin Koh
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iTRAQ8plex; Oxidation
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-26 02:42:22	ID requested
⏵ 1	2017-02-14 05:24:13	announced

Park YJ, Koh J, Kwon JT, Park YS, Yang L, Cha S, Uncovering stem cell differentiation factors for salivary gland regeneration by quantitative analysis of differential proteomes. PLoS One, 12(2):e0169677(2017) [pubmed]

curator keyword: Biological

submitter keyword: bone marrow-derived mesenchymal stem cells, salivary gland regeneration, iTRAQ, salivary epithelial precursors, co-culture, proteomics, transcription factors, dry mouth

Seunghee Cha
contact affiliation	University of Florida
contact email	scha@dental.ufl.edu
lab head
Jin Koh
contact affiliation	University of Florida
contact email	jinkoh@ufl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002796
     1. Label: PRIDE project
     2. Name: Quantitative Analysis of Differential Proteomes in Mouse Mesenchymal Stem Cells Converted to Salivary Epithelial Precursors