PXD002786 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Fusarium graminearum in vitro secretome |
Description | The plant pathogenic fungus Fusarium graminearum (Fgr) creates economic and health risks in cereals agriculture. Fgr causes head blight (or scab) of wheat and stalk rot of corn, reducing yield, degrading grain quality and polluting downstream food products with mycotoxins. Fungal plant pathogens must secrete proteases to access nutrition and to breakdown the structural protein component of the plant cell wall. Research into the proteolytic activity of Fgr is hindered by the complex nature of the suite of proteases secreted. We used a systems biology approach comprising genome analysis, transcriptomics and label-free quantitative proteomics to characterise the peptidases deployed by Fgr during growth. A combined analysis of published microarray transcriptome datasets revealed seven transcriptional groupings of peptidases based on in vitro growth, in planta growth, and sporulation behaviours. An orbitrap MS/MS proteomics technique defined the extracellular proteases secreted by Fusarium graminearum. |
HostingRepository | PRIDE |
AnnounceDate | 2015-10-06 |
AnnouncementXML | Submission_2016-03-31_01:27:33.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rohan Lowe |
SpeciesList | scientific name: Gibberella zeae (strain PH-1 / ATCC MYA-4620 / FGSC 9075 / NRRL 31084) (Wheat head blight fungus) (Fusarium graminearum); NCBI TaxID: 229533; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-08-25 01:10:37 | ID requested | |
1 | 2015-10-06 05:19:27 | announced | |
⏵ 2 | 2016-03-31 01:27:34 | announced | Updated publication reference for PubMed record(s): 26635820. |
Publication List
Lowe RG, McCorkelle O, Bleackley M, Collins C, Faou P, Mathivanan S, Anderson M, Extracellular peptidases of the cereal pathogen Fusarium graminearum. Front Plant Sci, 6():962(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Fusarium graminearum, secretome, peptidase, protease, in vitro. |
Contact List
Marilyn Anderson |
contact affiliation | Dept. Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Victoria 3086, Australia. |
contact email | m.anderson@latrobe.edu.au |
lab head | |
Rohan Lowe |
contact affiliation | La Trobe University |
contact email | r.lowe@latrobe.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002786
- Label: PRIDE project
- Name: Fusarium graminearum in vitro secretome