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PXD002786

PXD002786 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFusarium graminearum in vitro secretome
DescriptionThe plant pathogenic fungus Fusarium graminearum (Fgr) creates economic and health risks in cereals agriculture. Fgr causes head blight (or scab) of wheat and stalk rot of corn, reducing yield, degrading grain quality and polluting downstream food products with mycotoxins. Fungal plant pathogens must secrete proteases to access nutrition and to breakdown the structural protein component of the plant cell wall. Research into the proteolytic activity of Fgr is hindered by the complex nature of the suite of proteases secreted. We used a systems biology approach comprising genome analysis, transcriptomics and label-free quantitative proteomics to characterise the peptidases deployed by Fgr during growth. A combined analysis of published microarray transcriptome datasets revealed seven transcriptional groupings of peptidases based on in vitro growth, in planta growth, and sporulation behaviours. An orbitrap MS/MS proteomics technique defined the extracellular proteases secreted by Fusarium graminearum.
HostingRepositoryPRIDE
AnnounceDate2015-10-06
AnnouncementXMLSubmission_2016-03-31_01:27:33.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRohan Lowe
SpeciesList scientific name: Gibberella zeae (strain PH-1 / ATCC MYA-4620 / FGSC 9075 / NRRL 31084) (Wheat head blight fungus) (Fusarium graminearum); NCBI TaxID: 229533;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-08-25 01:10:37ID requested
12015-10-06 05:19:27announced
22016-03-31 01:27:34announcedUpdated publication reference for PubMed record(s): 26635820.
Publication List
Lowe RG, McCorkelle O, Bleackley M, Collins C, Faou P, Mathivanan S, Anderson M, Extracellular peptidases of the cereal pathogen Fusarium graminearum. Front Plant Sci, 6():962(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Fusarium graminearum, secretome, peptidase, protease, in vitro.
Contact List
Marilyn Anderson
contact affiliationDept. Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Victoria 3086, Australia.
contact emailm.anderson@latrobe.edu.au
lab head
Rohan Lowe
contact affiliationLa Trobe University
contact emailr.lowe@latrobe.edu.au
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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