PXD002783

PXD002783 is an original dataset announced via ProteomeXchange.

Title	Protein Profile Changes in the Frontotemporal Lobes in Human Severe Traumatic Brain Injury
Description	Severe traumatic brain injury (sTBI) is a serious public health issue with high morbidity and mortality rates. Previous proteomic studies on sTBI have mainly focused on human cerebrospinal fluid and serum, as well as on brain protein changes in murine models. However, human proteomic data in sTBI brain is still needed. We used proteomics and bioinformatics strategies to investigate variations in protein expression in human brains after sTBI, using samples from the Department of Neurosurgery, Affiliated Hospital of Hebei University (Hebei, P.R. China). Our proteomics data identified 4031 proteins, of which 162 proteins were overexpressed and 5 proteins were downregulated. The biological pathways that showed significant changes in protein expression according to bioinformatics analysis were glial cell differentiation, complement activation, apolipoprotein catalysis in statin pathway, and the blood coagulation cascade. Western blot verification of protein changes in a subset of the available tissue samples showed results that were consistent with the proteomics data. This study is one of the first to investigate the whole proteome of human sTBI brains, and provides a characteristic signature and overall landscape of the sTBI brain proteome.
HostingRepository	PRIDE
AnnounceDate	2016-04-15
AnnouncementXML	Submission_2016-04-15_06:35:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Ge
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-24 02:10:16	ID requested
⏵ 1	2016-04-15 06:35:57	announced

Xu B, Tian R, Wang X, Zhan S, Wang R, Guo Y, Ge W, Protein profile changes in the frontotemporal lobes in human severe traumatic brain injury. Brain Res, 1642():344-352(2016) [pubmed]

ProteomeXchange project tag: Human Proteome Project

curator keyword: Biomedical

submitter keyword: Severe traumatic brain injury

TMT (tandem mass tags)

human

proteomics

Wei Ge
contact affiliation	National Key Laboratory of Medical Molecular Biology and Department of Immunology; Institute of Basic Medical Sciences; Chinese Academy of Medical Sciences
contact email	wei.ge@chem.ox.ac.uk
lab head
Wei Ge
contact affiliation	National Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
contact email	wei.ge@chem.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD002783

PRIDE project URI

• PRIDE
  1. PXD002783
     1. Label: PRIDE project
     2. Name: Protein Profile Changes in the Frontotemporal Lobes in Human Severe Traumatic Brain Injury