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PXD002775

PXD002775 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExtrarenal α-klotho expression in human tissues
Descriptionα-Klotho has emerged as a powerful regulator of the ageing process. To-date, the expression profile of α-Klotho in human tissues is unknown and its existence in some human tissue types is subject to much controversy. Objective: This is the first study to characterize system-wide tissue expression of transmembrane α-Klotho in humans. We have employed next generation targeted proteomic analysis using Parallel Reaction Monitoring (PRM) in parallel with conventional antibody-based methods to determine the expression and spatial distribution of human α-Klotho expression in health. Results: The distribution of α-Klotho in human tissues from various organ systems, including arterial, epithelial, endocrine, reproductive and neuronal tissues was first identified by immunohistochemistry. Kidney tissues showed strong α-Klotho expression, while liver did not reveal a detectable signal. These results were next confirmed by western blotting of both whole tissues and primary cells. To validate our antibody-based results, α-Klotho expressing tissues were subjected to PRM mass spectrometry identifying peptides specific for the full length, transmembrane α-Klotho isoform. Conclusions: The data presented confirms α-Klotho expression in the kidney tubule and in artery, and provides evidence of α-Klotho expression across organ systems and cell-types that have not previously been described in humans.
HostingRepositoryPRIDE
AnnounceDate2015-10-13
AnnouncementXMLSubmission_2015-10-13_03:28:33.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLaurent Gatto
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-08-21 02:13:34ID requested
12015-10-13 03:28:35announced
Publication List
Lim K, Groen A, Molostvov G, Lu T, Lilley KS, Snead D, James S, Wilkinson IB, Ting S, Hsiao LL, Hiemstra TF, Zehnder D, -Klotho Expression in Human Tissues. J Clin Endocrinol Metab, 100(10):E1308-18(2015) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Human, Klotho, Ontogeny, PRM
Contact List
Kathryn Lilley
contact affiliationCambridge Centre for Proteomics University of Cambridge
contact emailksl23@cam.ac.uk
lab head
Laurent Gatto
contact affiliationDepartment of Biochemistry, University of Cambridge
contact emaillg390@cam.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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Repository Record List
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