PXD002775 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Extrarenal α-klotho expression in human tissues |
Description | α-Klotho has emerged as a powerful regulator of the ageing process. To-date, the expression profile of α-Klotho in human tissues is unknown and its existence in some human tissue types is subject to much controversy. Objective: This is the first study to characterize system-wide tissue expression of transmembrane α-Klotho in humans. We have employed next generation targeted proteomic analysis using Parallel Reaction Monitoring (PRM) in parallel with conventional antibody-based methods to determine the expression and spatial distribution of human α-Klotho expression in health. Results: The distribution of α-Klotho in human tissues from various organ systems, including arterial, epithelial, endocrine, reproductive and neuronal tissues was first identified by immunohistochemistry. Kidney tissues showed strong α-Klotho expression, while liver did not reveal a detectable signal. These results were next confirmed by western blotting of both whole tissues and primary cells. To validate our antibody-based results, α-Klotho expressing tissues were subjected to PRM mass spectrometry identifying peptides specific for the full length, transmembrane α-Klotho isoform. Conclusions: The data presented confirms α-Klotho expression in the kidney tubule and in artery, and provides evidence of α-Klotho expression across organ systems and cell-types that have not previously been described in humans. |
HostingRepository | PRIDE |
AnnounceDate | 2015-10-13 |
AnnouncementXML | Submission_2015-10-13_03:28:33.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Laurent Gatto |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-08-21 02:13:34 | ID requested | |
⏵ 1 | 2015-10-13 03:28:35 | announced | |
Publication List
Lim K, Groen A, Molostvov G, Lu T, Lilley KS, Snead D, James S, Wilkinson IB, Ting S, Hsiao LL, Hiemstra TF, Zehnder D, -Klotho Expression in Human Tissues. J Clin Endocrinol Metab, 100(10):E1308-18(2015) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Human, Klotho, Ontogeny, PRM |
Contact List
Kathryn Lilley |
contact affiliation | Cambridge Centre for Proteomics University of Cambridge |
contact email | ksl23@cam.ac.uk |
lab head | |
Laurent Gatto |
contact affiliation | Department of Biochemistry, University of Cambridge |
contact email | lg390@cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002775
- Label: PRIDE project
- Name: Extrarenal α-klotho expression in human tissues