PXD002768

PXD002768 is an original dataset announced via ProteomeXchange.

Title	Chicken spermatozoa protein extract analyzed by Top-down HRMS
Description	Currently, evaluation of quality semen is based on traditional in vitro sperm tests as measures of motility, viability and acrosomic reaction. However, the results are not always well correlated with real male fertility. In the present study, we evaluated Intact Cell MALDI-TOF Mass Spectrometry (ICM-MS) method as diagnostic molecular tool to substitute in vitro tests in order to phenotype semen with more robustness. For that, we investigated a large male population of known fertility that included two highly different genetic lines (meat and egg laying lines). Our optimized ICM-MS-based method through automation and the construction of fertility-predictive mathematical models was able to discriminate individuals on their reproductive capacity in the two lines studied. Furthermore, we showed a better diagnostic accuracy than traditional in vitro sperm quality tests and demonstrated that differential markers presenting a high discriminating power between fertile and subfertile sperm cells in sperm were implicated in fertility processes. To characterize m/z peaks observed in ICM-MS spectra, we performed high-throughput top-down protein identification of sperm cell extracts. Therefore, we identified more than 75% of the m/z peaks, revealing that these comprises mostly degradation products of testis-specific proteins implicated in the most important functional pathways in sperm cells such as energy metabolism and structure/movement.
HostingRepository	PRIDE
AnnounceDate	2016-04-11
AnnouncementXML	Submission_2016-04-11_03:00:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Labas Valerie
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	acetylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-20 03:53:12	ID requested
⏵ 1	2016-04-11 03:00:31	announced

Soler L, Labas V, Th, é, lie A, Grasseau I, Teixeira-Gomes AP, Blesbois E, Intact Cell MALDI-TOF MS on Sperm: A Molecular Test For Male Fertility Diagnosis. Mol Cell Proteomics, 15(6):1998-2010(2016) [pubmed]

curator keyword: Biological

submitter keyword: Chicken, spermatozoa, top-down

PAIB INRA platform, Mass spectrometry Laboratory, Valérie Labas
contact affiliation	INRA Centre val de Loire 5tours, France) UMR INRA 85 CNRS 7247 UFR IFCE Physiologie de la reproduction et des comportements PAIB2 platform
contact email	valerie.labas@tours.inra.fr
lab head
Labas Valerie
contact affiliation	INRA-Nouzilly-PRC
contact email	valerie.labas@tours.inra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD002768

PRIDE project URI

• PRIDE
  1. PXD002768
     1. Label: PRIDE project
     2. Name: Chicken spermatozoa protein extract analyzed by Top-down HRMS