PXD002767

PXD002767 is an original dataset announced via ProteomeXchange.

Title	Time-course Investigation of Phytophthora infestans infection of potato leaf from three cultivars by quantitative proteomics
Description	Potato Late blight is one the most important crop diseases worldwide. Even though potato has been studied for many years, the potato disease late blight still has a huge negative effect on the potato production. A total of three commercially available field potato cultivars of different resistance to late blight infection: Kuras (moderate), Sarpo Mira (highly resistant) and Bintje (very suseptable) under controlled green house growing conditions innoculated with a diversity of P. infestans populations. We used label-free quantitative proteomics to investigate the infection with P. infestans in a time-course study over 258 hours. Several key issues limits proteome analysis of potato leaf tissue4–6. Firstly, the immense complexity of the plant proteome which is further complicated by the presence of highly abundant proteins, such as ribulose bisphosphate carboxylase/oxygenase (RuBisCO). Secondly, plant leaf and potato in particular contain abundant levels amounts of phenols and polyphenols which hinder or, unless precautions are taken, completely prevent a successful protein extraction.
HostingRepository	PRIDE
AnnounceDate	2016-01-05
AnnouncementXML	Submission_2016-03-16_06:19:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Allan Stensballe
SpeciesList	scientific name: Solanum tuberosum (Potato); NCBI TaxID: 4113;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-20 01:00:42	ID requested
1	2016-01-05 10:33:58	announced
⏵ 2	2016-03-16 06:19:31	announced	Updated publication reference for PubMed record(s): 26862565.

Larsen MK, J, ø, rgensen MM, Bennike TB, Stensballe A, Time-course investigation of Phytophthora infestans infection of potato leaf from three cultivars by quantitative proteomics. Data Brief, 6():238-48(2016) [pubmed]

curator keyword: Biological

submitter keyword: Proteomics, potato, Phytophthora infestans, late blight, leaf

Allan Stensballe
contact affiliation	Aalborg University Fredrik Bajersvej 7E 9220 Aalborg Denmark
contact email	as@hst.aau.dk
lab head
Allan Stensballe
contact affiliation	Aalborg University
contact email	as@hst.aau.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002767

PRIDE project URI

• PRIDE
  1. PXD002767
     1. Label: PRIDE project
     2. Name: Time-course Investigation of Phytophthora infestans infection of potato leaf from three cultivars by quantitative proteomics