PXD002743

PXD002743 is an original dataset announced via ProteomeXchange.

Title	DUSP2 dephosphorylates STAT3 at Y705 and S727 residues
Description	In the related study, to determine whether DUSP2 definitively served as a phosphatase for STAT3, an in vitro phosphatase assay was used. Using S-tag beads, p-STAT3 was pulled down from IL-6 stimulated HEK293T cells transfected with STAT3-S-Tag. DUSP2 or control protein, which was purified from HEK293T cells transfected with DUSP2-FLAG or empty vector through extraction with anti-FLAG beads and elution with FLAG peptides, were incubated with p-STAT3-S-Tag-beads in phosphatase buffer. Then bound proteins were eluted and subjected to MS analysis. When compared with the control, incubation of STAT3 and DUSP2 led to dephosphorylation of two STAT3 residues that have been reported to promote its activity, namely tyrosine 705 and serine 727.
HostingRepository	PRIDE
AnnounceDate	2015-10-13
AnnouncementXML	Submission_2016-03-31_01:25:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dan Lu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-17 08:42:58	ID requested
1	2015-10-13 08:34:01	announced
⏵ 2	2016-03-31 01:25:20	announced	Updated publication reference for PubMed record(s): 26479789.

Lu D, Liu L, Ji X, Gao Y, Chen X, Liu Y, Liu Y, Zhao X, Li Y, Li Y, Jin Y, Zhang Y, McNutt MA, Yin Y, The phosphatase DUSP2 controls the activity of the transcription activator STAT3 and regulates TH17 differentiation. Nat Immunol, 16(12):1263-73(2015) [pubmed]

curator keyword: Biological

submitter keyword: DUSP2, STAT3, phosphorylation

Yuxin Yin
contact affiliation	Institute of Systems Biomedicine, Peking University Health Science Center
contact email	yinyuxin@hsc.pku.edu.cn
lab head
Dan Lu
contact affiliation	Peking University Health Science Center
contact email	danlu_pku@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002743
     1. Label: PRIDE project
     2. Name: DUSP2 dephosphorylates STAT3 at Y705 and S727 residues