PXD002707

PXD002707 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS analysis of IαI proteoglycopeptides
Description	The inter-alpha-trypsin inhibitor (IαI) complex is a macromolecular arrangement of structurally related heavy chain proteins covalently cross-linked to the chondroitin sulfate (CS) chain of the proteoglycan bikunin. The IαI complex is abundant in plasma and associated with inflammation, kidney diseases, cancer and diabetes. Bikunin is modified at Ser-10 by a single low-sulfated CS chain of 23-55 monosaccharides with 4-9 sulfate groups. The innermost four monosaccharides (GlcA3Gal3Gal4Xyl-O-) compose the linkage region, believed to be uniform with a 4-O-sulfation to the outer Gal. The cross-linkage region of the bikunin CS chain is located in the non-sulfated non-reducing end, (GalNAc4GlcA3)n ,to which heavy chains (H1-H3) may be bound in GalNAc to Asp ester linkages. In this study we employed a glycoproteomics protocol to enrich and analyze light and heavy chain linkage and cross-linkage region CS glycopeptides derived from the IαI complex of human plasma, urine and cerebrospinal fluid samples. The samples were trypsinized, enriched by strong anion exchange chromatography, partially depolymerized with chondroitinase ABC and analyzed by LC-MS/MS using higher-energy collisional dissociation (HCD). The analyses demonstrated that the CS linkage region of bikunin is highly heterogeneous. In addition to sulfation of the Gal residue, Xyl phosphorylation was observed although exclusively in urinary samples. We also identified novel Neu5Ac and Fuc modifications of the linkage region as well as the presence of mono- and disialylated core 1 O-linked glycans on Thr-17. Heavy chains H1 and H2 were identified cross-linked to GalNAc residues one or two GlcA residues apart and H1 was found linked to either the terminal or subterminal two GalNAc residues. The fragmentation behavior of CS glycopeptides under variable HCD conditions displays an energy dependency that may be used to obtain complementary structural details. Finally, we show that the analysis of sodium adducts provides confirmatory information about the positions of glycan substituents.
HostingRepository	PRIDE
AnnounceDate	2015-10-02
AnnouncementXML	Submission_2015-10-02_05:23:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alejandro Gómez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-12 06:46:48	ID requested
⏵ 1	2015-10-02 05:23:54	announced

Gomez Toledo A, Nilsson J, Noborn F, Sihlbom C, Larson G, -Trypsin Inhibitor Complex. Mol Cell Proteomics, 14(12):3118-31(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: proteoglycan, protein AMBP, chondroitin sulfate, linkage region, heavy chain, mass spectrometry

Göran Larson
contact affiliation	Sahlgrenska Academy, University of Gothenburg
contact email	goran.larson@clinchem.gu.se
lab head
Alejandro Gómez
contact affiliation	University of Gothenburg
contact email	alejandro.gomez.toledo@gu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002707
     1. Label: PRIDE project
     2. Name: LC-MS/MS analysis of IαI proteoglycopeptides