PXD002706

PXD002706 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Protein tyrosine kinases analysis by multiplexed parallel reaction monitoring assays
Description	In this study, we have developed a parallel reaction monitoring (PRM)-based assay for quantitative profiling of 83 PTKs. The assay detects 308 proteotypic peptides from 54 receptor tyrosine kinases and 29 nonreceptor tyrosine kinases in a single run. We used this assay to determine PTKs in lung cancer cell lines with either susceptibility (11-18) or acquired resistance (11-18R) to the epidermal growth factor receptor tyrosine kinase inhibitor erlotinib. We also performed label free shotgun proteome analyses of the 11-18 and 11-18R cells and iTRAQ comparisons of phosphotyrosine peptides from 11-18 and 11-18R cells.
HostingRepository	MassIVE
AnnounceDate	2019-10-03
AnnouncementXML	Submission_2019-10-03_08:36:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robbert Slebos
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	O4'-phospho-L-tyrosine
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-12 06:00:33	ID requested
⏵ 1	2019-10-03 08:36:41	announced

Publication List

Kim HJ, Lin D, Lee HJ, Li M, Liebler DC, Quantitative Profiling of Protein Tyrosine Kinases in Human Cancer Cell Lines by Multiplexed Parallel Reaction Monitoring Assays. Mol Cell Proteomics, 15(2):682-91(2016) [pubmed]

Kim HJ, Lin D, Lee HJ, Li M, Liebler DC, Quantitative Profiling of Protein Tyrosine Kinases in Human Cancer Cell Lines by Multiplexed Parallel Reaction Monitoring Assays. Mol Cell Proteomics, 15(2):682-91(2016) [pubmed]

Keyword List

submitter keyword: receptor tyrosine kinase

submitter keyword: receptor tyrosine kinase

Contact List

Daniel Liebler
lab head
Robbert Slebos
contact affiliation	Moffitt
contact email	robbert.slebos@moffitt.org
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079224/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079224/