PXD002691

PXD002691 is an original dataset announced via ProteomeXchange.

Title	Autophosphorylation of Entamoeba histolytica atypical kinase EhAK1
Description	Phagocytosis plays a key role in survival and pathogenicity of Entamoeba histolytica. We have recently demonstrated that an atypical kinase EhAK1 is involved in phagocytosis in this parasite. It is recruited to the phagocytic cups through interaction with EhCaBP1. EhAK1 manipulates actin dynamics by multiple mechanisms including phosphorylation of G-actin. Biochemical analysis showed that EhAK1 is a serine/threonine kinase with broad ion specificity and undergoes multiple trans-autophosphorylation. Multiple autophosphorylation sites were identified by mass spectrometry. Out of these Thr279 appears to be involved in both autophosphorylation as well as substrate phosphorylation.
HostingRepository	PRIDE
AnnounceDate	2018-10-24
AnnouncementXML	Submission_2018-10-24_10:50:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	gagan jhingan
SpeciesList	scientific name: Entamoeba histolytica HM-1:IMSS-A; NCBI TaxID: 885318;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-10 03:24:56	ID requested
⏵ 1	2018-10-24 10:50:47	announced

Mansuri MS, Babuta M, Ali MS, Bharadwaj R, Deep jhingan G, Gourinath S, Bhattacharya S, Bhattacharya A, Autophosphorylation at Thr279 of Entamoeba histolytica atypical kinase EhAK1 is required for activity and regulation of erythrophagocytosis. Sci Rep, 6():16969(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: kinase, entamoeba, proteomics, phagocytosis

ALOK BHATTACHARYA
contact affiliation	School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067
contact email	alok0200@mail.jnu.ac.in
lab head
gagan jhingan
contact affiliation	NII
contact email	gaganjhingan@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002691
     1. Label: PRIDE project
     2. Name: Autophosphorylation of Entamoeba histolytica atypical kinase EhAK1