PXD002690

PXD002690 is an original dataset announced via ProteomeXchange.

Title	Large scale profiling of the physiological impact of a potent anti-cancer agent inhibiting early protein maturation
Description	Fumagillin and its derivatives are therapeutically-useful compounds for their capacity to reduce cancer progression. Fumagillin exerts a specific proliferation inhibition on endothelial cell lines and on several tumor lines. The specific molecular target of fumagillin is MetAP2, one of the two cytosolic MetAPs. MetAPs are in charge of N-terminal Methionine Excision, an essential pathway of cotranslational protein maturation. Why the inhibition of MetAP2 causes cell growth arrest in a subset class of cells is yet unknown. Here, we focus on a global large scale characterization of the N-terminal Methionine Excision pathway and the inhibition of one of its enzymes by fumagillin in a number of lines including cancer cell lines. N-termini profiling of responsive and unresponsive cells to fumagillin treatment was conducted using large scale proteomics approach
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_03:58:31.131.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Willy Bienvenut
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	(R)-5-oxo-1; potassium containing modified residue; acetate labeling reagent (N-term) (heavy form, +3amu); 3x(2)H labeled N6-acetyl-L-lysine; phosphorylated residue; mono N-acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-10 02:12:40	ID requested
1	2017-01-02 07:02:30	announced
2	2017-10-24 04:12:15	announced	Updated project metadata.
⏵ 3	2024-10-22 03:58:37	announced	2024-10-22: Updated project metadata.

10.18632/oncotarget.11216;

Frottin F, Bienvenut WV, Bignon J, Jacquet E, Vaca Jacome AS, Van Dorsselaer A, Cianferani S, Carapito C, Meinnel T, Giglione C, MetAP1 and MetAP2 drive cell selectivity for a potent anti-cancer agent in synergy, by controlling glutathione redox state. Oncotarget, 7(39):63306-63323(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: co-translational modifications

N-terminal processing

methionine aminopeptidase

N-α-acetyltransferase

N-myristoyltransferase

drug evaluation;

Willy V Bienvenut
contact affiliation	Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Avenue de la Terrasse, F-91198 Gif-sur-Yvette cedex, France
contact email	willy.bienvenut@i2bc.paris-saclay.fr
lab head
Willy Bienvenut
contact affiliation	CNRS
contact email	willy.bienvenut@universite-paris-saclay.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD002690

PRIDE project URI

• PRIDE
  1. PXD002690
     1. Label: PRIDE project
     2. Name: Large scale profiling of the physiological impact of a potent anti-cancer agent inhibiting early protein maturation