PXD002688 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Systematic Evaluation of Data-Independent Acquisition for Sensitive and Reproducible Proteomics – a Prototype Design for a Single Injection Assay |
Description | This dataset includes twelve DIA methods with various mass-isolation windows that analyze precursor ranges from 400-1250 m/z as well as shotgun acquired data with the same instrument and LC gradient. The mass-isolation windows analyzed included 3, 6, and 26 m/z widths. Each 3 m/z window method analyzed a 140 precursor m/z range, 6 m/z windows analyzed either a 200 or 280 precursor m/z range, and the 26 m/z isolation window method analyzed an 800 precursor m/z range from 400-1200 m/z. All data were collected with a 5600+ Sciex QTOF with a 70 minute reverse phase gradient. Shotgun analyses were collected with the sample samples, instrument, and gradient. |
HostingRepository | PRIDE |
AnnounceDate | 2015-12-02 |
AnnouncementXML | Submission_2016-01-18_05:03:36.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Michael Heaven |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | deamidated residue; monohydroxylated residue; acetylated residue; phosphorylated residue; iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-08-10 02:02:27 | ID requested | |
1 | 2015-12-02 08:05:38 | announced | |
⏵ 2 | 2016-01-18 05:03:37 | announced | Updated publication reference for PubMed record(s): 26757066. |
Publication List
Heaven MR, Funk AJ, Cobbs AL, Haffey WD, Norris JL, McCullumsmith RE, Greis KD, Systematic evaluation of data-independent acquisition for sensitive and reproducible proteomics-a prototype design for a single injection assay. J Mass Spectrom, 51(1):1-11(2016) [pubmed] |
Keyword List
curator keyword: Technical |
submitter keyword: Protalizer |
DIA |
SWATH |
PAcIFIC |
label-free quantification |
Contact List
Kenneth D. Greis |
contact affiliation | Department of Cancer Biology, University of Cincinnati College of Medicine |
contact email | greiskd@ucmail.uc.edu |
lab head | |
Michael Heaven |
contact affiliation | Vulcan Analytical |
contact email | mike@vulcan.ms |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/12/PXD002688 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002688
- Label: PRIDE project
- Name: Systematic Evaluation of Data-Independent Acquisition for Sensitive and Reproducible Proteomics – a Prototype Design for a Single Injection Assay