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PXD002688

PXD002688 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSystematic Evaluation of Data-Independent Acquisition for Sensitive and Reproducible Proteomics – a Prototype Design for a Single Injection Assay
DescriptionThis dataset includes twelve DIA methods with various mass-isolation windows that analyze precursor ranges from 400-1250 m/z as well as shotgun acquired data with the same instrument and LC gradient. The mass-isolation windows analyzed included 3, 6, and 26 m/z widths. Each 3 m/z window method analyzed a 140 precursor m/z range, 6 m/z windows analyzed either a 200 or 280 precursor m/z range, and the 26 m/z isolation window method analyzed an 800 precursor m/z range from 400-1200 m/z. All data were collected with a 5600+ Sciex QTOF with a 70 minute reverse phase gradient. Shotgun analyses were collected with the sample samples, instrument, and gradient.
HostingRepositoryPRIDE
AnnounceDate2015-12-02
AnnouncementXMLSubmission_2016-01-18_05:03:36.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMichael Heaven
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListdeamidated residue; monohydroxylated residue; acetylated residue; phosphorylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-08-10 02:02:27ID requested
12015-12-02 08:05:38announced
22016-01-18 05:03:37announcedUpdated publication reference for PubMed record(s): 26757066.
Publication List
Heaven MR, Funk AJ, Cobbs AL, Haffey WD, Norris JL, McCullumsmith RE, Greis KD, Systematic evaluation of data-independent acquisition for sensitive and reproducible proteomics-a prototype design for a single injection assay. J Mass Spectrom, 51(1):1-11(2016) [pubmed]
Keyword List
curator keyword: Technical
submitter keyword: Protalizer
DIA
SWATH
PAcIFIC
label-free quantification
Contact List
Kenneth D. Greis
contact affiliationDepartment of Cancer Biology, University of Cincinnati College of Medicine
contact emailgreiskd@ucmail.uc.edu
lab head
Michael Heaven
contact affiliationVulcan Analytical
contact emailmike@vulcan.ms
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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