PXD002681 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cryo-slicing BN-MS of rat brain mitochondrial proteins |
Description | Blue-native gel electrophoresis (BN) is a powerful method for protein separation. Combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) it enables large-scale identification of protein complexes and their subunits. Current BN-MS approaches, however, are limited in size resolution, comprehensiveness and quantification. Here, we present a new methodology combining defined sub-mm slicing of BN gels by a cryo-microtome with high-performance LC-MS/MS and label-free quantification of proteins. Application of this cryo-slicing BN-MS approach (csBN-MS) to mitochondria from brain demonstrated a high degree of comprehensiveness, quantification accuracy and size resolution. The technique provided abundance-mass profiles for 743 mitochondrial proteins including all canonical subunits of the oxidative respiratory chain assembled into 13 distinct (super)complexes. Moreover, the data revealed COX7R as a constitutive subunit of distinct supercomplexes and identified novel assemblies of porins and TOM proteins. Together, csBN-MS enables quantitative profiling of complexomes with resolution close to the limits of native gel electrophoresis. |
HostingRepository | PRIDE |
AnnounceDate | 2015-12-01 |
AnnouncementXML | Submission_2015-12-01_07:00:50.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD002681 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Alexander Haupt |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | Oxidation; Gln->pyro-Glu; Propionamide; Glu->pyro-Glu; Acetyl; Carbamidomethyl |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-08-07 07:45:54 | ID requested | |
⏵ 1 | 2015-12-01 07:00:51 | announced | |
Publication List
M, ΓΌ, ller CS, Bildl W, Haupt A, Ellenrieder L, Becker T, Hunte C, Fakler B, Schulte U, Cryo-slicing Blue Native-Mass Spectrometry (csBN-MS), a Novel Technology for High Resolution Complexome Profiling. Mol Cell Proteomics, 15(2):669-81(2016) [pubmed] |
Keyword List
curator keyword: Biomedical, Biological |
submitter keyword: cryo-slicing BN-MS LC-MS/MS complexome proteine profiles |
Contact List
Prof. Dr. Bernd Fakler |
contact affiliation | Institute of Physiology II, University of Freiburg, Germany |
contact email | bernd.fakler@physiologie.uni-freiburg.de |
lab head | |
Alexander Haupt |
contact affiliation | University of Freiburg, Germany |
contact email | alexander.haupt@physiologie.uni-freiburg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002681
- Label: PRIDE project
- Name: Cryo-slicing BN-MS of rat brain mitochondrial proteins