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PXD002681

PXD002681 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCryo-slicing BN-MS of rat brain mitochondrial proteins
DescriptionBlue-native gel electrophoresis (BN) is a powerful method for protein separation. Combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) it enables large-scale identification of protein complexes and their subunits. Current BN-MS approaches, however, are limited in size resolution, comprehensiveness and quantification. Here, we present a new methodology combining defined sub-mm slicing of BN gels by a cryo-microtome with high-performance LC-MS/MS and label-free quantification of proteins. Application of this cryo-slicing BN-MS approach (csBN-MS) to mitochondria from brain demonstrated a high degree of comprehensiveness, quantification accuracy and size resolution. The technique provided abundance-mass profiles for 743 mitochondrial proteins including all canonical subunits of the oxidative respiratory chain assembled into 13 distinct (super)complexes. Moreover, the data revealed COX7R as a constitutive subunit of distinct supercomplexes and identified novel assemblies of porins and TOM proteins. Together, csBN-MS enables quantitative profiling of complexomes with resolution close to the limits of native gel electrophoresis.
HostingRepositoryPRIDE
AnnounceDate2015-12-01
AnnouncementXMLSubmission_2015-12-01_07:00:50.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD002681
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlexander Haupt
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListOxidation; Gln->pyro-Glu; Propionamide; Glu->pyro-Glu; Acetyl; Carbamidomethyl
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-08-07 07:45:54ID requested
12015-12-01 07:00:51announced
Publication List
M, ΓΌ, ller CS, Bildl W, Haupt A, Ellenrieder L, Becker T, Hunte C, Fakler B, Schulte U, Cryo-slicing Blue Native-Mass Spectrometry (csBN-MS), a Novel Technology for High Resolution Complexome Profiling. Mol Cell Proteomics, 15(2):669-81(2016) [pubmed]
Keyword List
curator keyword: Biomedical, Biological
submitter keyword: cryo-slicing BN-MS LC-MS/MS complexome proteine profiles
Contact List
Prof. Dr. Bernd Fakler
contact affiliationInstitute of Physiology II, University of Freiburg, Germany
contact emailbernd.fakler@physiologie.uni-freiburg.de
lab head
Alexander Haupt
contact affiliationUniversity of Freiburg, Germany
contact emailalexander.haupt@physiologie.uni-freiburg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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