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PXD002678

PXD002678 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGluten-specific antibodies of celiac disease gut plasma cells recognize long proteolytic fragments that typically harbor T-cell epitopes
DescriptionThis study aimed to identify proteolytic fragments of gluten proteins recognized by recombinant IgG1 monoclonal antibodies generated from single IgA plasma cells of celiac disease lesions. Peptides bound by monoclonal antibodies in complex gut-enzyme digests of gluten treated with the deamidating enzyme transglutaminase 2, were identified by mass spectrometry after antibody pull-down with protein G beads. The antibody bound peptides were long deamidated peptide fragments that contained the substrate recognition sequence of transglutaminase 2. Characteristically, the fragments contained epitopes with the sequence QPEQPFP and variants thereof in multiple copies, and they typically also harbored many different gluten T-cell epitopes. In the pull-down setting where antibodies were immobilized on a solid phase, peptide fragments with multivalent display of epitopes were targeted. This scenario resembles the situation of the B-cell receptor on the surface of B cells. Conceivably, B cells of celiac disease patients select gluten epitopes that are repeated multiple times in long peptide fragments generated by gut digestive enzymes. As the fragments also contain many different T-cell epitopes, this will lead to generation of strong antibody responses by effective presentation of several distinct T-cell epitopes and establishment of T-cell help to B cells.
HostingRepositoryPRIDE
AnnounceDate2016-05-10
AnnouncementXMLSubmission_2016-05-10_04:42:27.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSiri Dørum
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; deamidated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-08-06 02:40:09ID requested
12016-05-10 04:42:28announced
Publication List
D, ø, rum S, Steinsb, ø Ø, Bergseng E, Arntzen M Ø, de Souza GA, Sollid LM, Gluten-specific antibodies of celiac disease gut plasma cells recognize long proteolytic fragments that typically harbor T-cell epitopes. Sci Rep, 6():25565(2016) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Celiac disease, gliadin, monoclonal antibodies, epitopes, LC-MSMS
Contact List
Gustavo A. de Souza
contact affiliation1. Centre for Immune Regulation and Department of Immunology, University of Oslo and Oslo University Hospital - Rikshospitalet, 0424 Oslo, Norway 2. Proteomics Core Facility, Oslo University Hospital-Rikshospitalet, 0424 Oslo, Norway
contact emailg.a.d.souza@medisin.uio.no
lab head
Siri Dørum
contact affiliationCentre for Immune Regulation, University of Oslo
contact emailsiri.dorum@rr-research.no
dataset submitter
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Dataset FTP location
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