PXD002668

PXD002668 is an original dataset announced via ProteomeXchange.

Title	Isolation of extracellular vesicles by PROSPR
Description	Here, we report a novel and inexpensive method of rapidly isolating EVs from small volumes of human blood plasma by PRotein Organic Solvent PRecipitation (PROSPR). PROSPR encompasses a rapid three-step protocol to remove soluble proteins from plasma via precipitation in cold acetone, leaving the lipid-encapsulated EVs behind in suspension. This generates higher purity EVs that can then be obtained from filtration or classical ultracentrifugation methods.
HostingRepository	PRIDE
AnnounceDate	2015-10-05
AnnouncementXML	Submission_2015-10-05_04:32:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xavier Gallart Palau
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-05 02:35:56	ID requested
⏵ 1	2015-10-05 04:32:13	announced

Gallart-Palau X, Serra A, Wong AS, Sandin S, Lai MK, Chen CP, Kon OL, Sze SK, Extracellular vesicles are rapidly purified from human plasma by PRotein Organic Solvent PRecipitation (PROSPR). Sci Rep, 5():14664(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Extracellular vesicles, Exosome, Blood plasma, PROSPR, Biomarker, Biochemical Purification

Siu Kwan Sze
contact affiliation	School of Biological Sciences Division of Chemical Biology & BioTechnology Nanyang Technological University, 60 Nanyang Drive, Singapore 637551
contact email	SKSze@ntu.edu.sg
lab head
Xavier Gallart Palau
contact affiliation	Reasearch Associate
contact email	xgallart@ntu.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/10/PXD002668

PRIDE project URI

• PRIDE
  1. PXD002668
     1. Label: PRIDE project
     2. Name: Isolation of extracellular vesicles by PROSPR