PXD002662

PXD002662 is an original dataset announced via ProteomeXchange.

Title	Molecular responses of alveolar epithelial A549 cells to chronic exposure to titanium dioxide nanoparticles: a proteomic view
Description	Although the biological effects of titanium dioxide nanoparticles (TiO2-NPs) have been studied for more than two decades, the mechanisms governing their toxicity are still unclear. We applied 2D-gel proteomics analysis on A549 epithelial alveolar cells chronically exposed for 2 months to 2.5 or 50 µg/mL of deeply characterized TiO2-NPs, in order to obtain comprehensive molecular responses that may reflect functional outcomes. We show that exposure to TiO2-NPs impacts the abundance of 30 protein species, corresponding to 22 gene products. These proteins are involved in glucose metabolism, trafficking, gene expression, mitochondrial function, proteasome activity and DNA damage response. Besides, our results suggest that p53 pathway is activated, slowing down cell cycle progression and reducing cell proliferation rate. Moreover, we report increased content of chaperones-related proteins, which suggests homeostasis re-establishment. Finally, our results highlight that chronic exposure to TiO2-NPs affects the same cellular functions as acute exposure to TiO2-NPs, although lower exposure concentrations and longer exposure times induce more intense cellular response.
HostingRepository	PRIDE
AnnounceDate	2015-08-24
AnnouncementXML	Submission_2015-08-24_03:40:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jean-Marc STRUB
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-04 05:53:03	ID requested
⏵ 1	2015-08-24 03:40:05	announced

Armand L, Biola-Clier M, Bobyk L, Collin-Faure V, Diemer H, Strub JM, Cianferani S, Van Dorsselaer A, Herlin-Boime N, Rabilloud T, Carriere M, Molecular responses of alveolar epithelial A549 cells to chronic exposure to titanium dioxide nanoparticles: A proteomic view. J Proteomics, 134():163-173(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: titanium dioxide nanoparticles, 2D gel electrophoresis, nanoLC-MS/MS

Thierry, Rabilloud
contact affiliation	CNRS UMR 5249, Laboratory of Chemistry and Biology of Metals, Grenoble, France
contact email	thierry.rabilloud@cnrs.fr
lab head
Jean-Marc STRUB
contact affiliation	CNRS
contact email	jmstrub@unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002662
     1. Label: PRIDE project
     2. Name: Molecular responses of alveolar epithelial A549 cells to chronic exposure to titanium dioxide nanoparticles: a proteomic view