PXD002661

PXD002661 is an original dataset announced via ProteomeXchange.

Title	Global Metabonomic and Proteomic Analysis of Human Conjunctival Epithelial Cells (IOBA-NHC) in Response to Hyperosmotic Stress
Description	“Dry eye” is a multi-factorial inflammatory disease affecting the ocular surface. Tear hyperosmolarity in dry eye contributes to inflammation and cell damage. Recent research efforts on dry eye have been directed towards biomarker discovery for diagnosis, response to treatment and disease mechanisms. This study employed a spontaneously immortalized normal human conjunctival cell line, IOBA-NHC as a model to investigate hyperosmotic stress-induced changes of metabolites and proteins. Global and targeted metabonomic analyses, as well as proteomic analysis were performed on IOBA-NHC cells incubated in serum-free media at 280 mOsm (control), 380 mOsm and 480 mOsm for 24 h. Twenty-one metabolites and seventy-six iTRAQ-identified proteins showed significant changes under at least one hyperosmotic stress treatment as compared to controls. SWATH-based proteomic analysis further confirmed the involvement of inflammatory pathways such as prostaglandin 2 synthesis in IOBA-NHC cells under hyperosmotic stress. This study is the first to identify glycerophosphocholine synthesis and O-linked β-N-acetylglucosamine glycosylation as key activated pathways in ocular surface cells under hyperosmotic stress. These findings extend the current knowledge in metabolite markers of dry eye and provide potential therapeutic targets for its treatment.
HostingRepository	PRIDE
AnnounceDate	2015-08-17
AnnouncementXML	Submission_2015-08-17_02:59:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liyan Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-04 04:03:09	ID requested
⏵ 1	2015-08-17 02:59:07	announced

Chen L, Li J, Guo T, Ghosh S, Koh SK, Tian D, Zhang L, Jia D, Beuerman RW, Aebersold R, Chan EC, Zhou L, Global Metabonomic and Proteomic Analysis of Human Conjunctival Epithelial Cells (IOBA-NHC) in Response to Hyperosmotic Stress. J Proteome Res, 14(9):3982-95(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Dry eye, osmostic stress, hyperosmolarity, osmoprotectants, metabolomics, metabonomics, proteomics, iTRAQ, SWATH

Lei Zhou
contact affiliation	Senior Principal Research Scientist, Head, Ocular Proteomics Platform Singapore Eye Research Institute Singapore
contact email	zhou.lei@seri.com.sg
lab head
Liyan Chen
contact affiliation	Singapore Eye Research Institute
contact email	liyan.chen.sg@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002661
     1. Label: PRIDE project
     2. Name: Global Metabonomic and Proteomic Analysis of Human Conjunctival Epithelial Cells (IOBA-NHC) in Response to Hyperosmotic Stress