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PXD002656

PXD002656 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDithiothreitol (DTT) acts as a specific, UV-inducible cross-linker in elucidation of protein-RNA interactions
DescriptionIn the present paper, we introduce dithiothreitol (DTT) as a potent protein-RNA cross-linker. To prove this three model systems, a) a small synthetic peptide from smB'/B protein incubated with U1snRNA oligonucleotide, b) in vitro reconstituted 15.5K protein with U4 snRNA oligonucleotide and c) native ribonucleoprotein-complexes (RNPs) from S. cerevisiae were used . All protein-RNA complexes were UV irradiated and heteroconjugates cross-linked enriched prior to LC-MS analysis. Our results unambiguously show that DTT covalently participates in cysteine-uracil crosslinks which is observable as a mass increments of 152 Da upon mass spectrometric analysis.
HostingRepositoryPRIDE
AnnounceDate2015-10-13
AnnouncementXMLSubmission_2015-10-13_00:36:04.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterUzma Zaman
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive; LTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-08-03 06:56:13ID requested
12015-10-13 00:36:06announced
Publication List
Zaman U, Richter FM, Hofele R, Kramer K, Sachsenberg T, Kohlbacher O, Lenz C, Urlaub H, Dithiothreitol (DTT) Acts as a Specific, UV-inducible Cross-linker in Elucidation of Protein-RNA Interactions. Mol Cell Proteomics, 14(12):3196-210(2015) [pubmed]
Keyword List
submitter keyword: DTT
Contact List
Henning Urlaub
contact affiliationBioanalytical Mass spectrometry, Max Planck Institute For Biophysical Chemistry, Goettingen, Germany
contact emailhenning.urlaub@mpibpc.mpg.de
lab head
Uzma Zaman
contact affiliationMax Planck Institute for Biophysical Chemistry
contact emailuzma.zaman@mpibpc.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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