PXD002656 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dithiothreitol (DTT) acts as a specific, UV-inducible cross-linker in elucidation of protein-RNA interactions |
Description | In the present paper, we introduce dithiothreitol (DTT) as a potent protein-RNA cross-linker. To prove this three model systems, a) a small synthetic peptide from smB'/B protein incubated with U1snRNA oligonucleotide, b) in vitro reconstituted 15.5K protein with U4 snRNA oligonucleotide and c) native ribonucleoprotein-complexes (RNPs) from S. cerevisiae were used . All protein-RNA complexes were UV irradiated and heteroconjugates cross-linked enriched prior to LC-MS analysis. Our results unambiguously show that DTT covalently participates in cysteine-uracil crosslinks which is observable as a mass increments of 152 Da upon mass spectrometric analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2015-10-13 |
AnnouncementXML | Submission_2015-10-13_00:36:04.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Uzma Zaman |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive; LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-08-03 06:56:13 | ID requested | |
⏵ 1 | 2015-10-13 00:36:06 | announced | |
Publication List
Zaman U, Richter FM, Hofele R, Kramer K, Sachsenberg T, Kohlbacher O, Lenz C, Urlaub H, Dithiothreitol (DTT) Acts as a Specific, UV-inducible Cross-linker in Elucidation of Protein-RNA Interactions. Mol Cell Proteomics, 14(12):3196-210(2015) [pubmed] |
Keyword List
Contact List
Henning Urlaub |
contact affiliation | Bioanalytical Mass spectrometry, Max Planck Institute For Biophysical Chemistry, Goettingen, Germany |
contact email | henning.urlaub@mpibpc.mpg.de |
lab head | |
Uzma Zaman |
contact affiliation | Max Planck Institute for Biophysical Chemistry |
contact email | uzma.zaman@mpibpc.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/10/PXD002656 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002656
- Label: PRIDE project
- Name: Dithiothreitol (DTT) acts as a specific, UV-inducible cross-linker in elucidation of protein-RNA interactions