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PXD002646

PXD002646 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA systematic evaluation of nanocast metal oxide microspheres for phosphoproteomics applications
DescriptionWe used the nanocasting technique to prepare a range of different metal oxide microspheres that allow an objective comparison of the different materials. Using samples of different complexity, ranging from synthetic peptides to whole cell lysates, we characterized the binding behavior of these materials. We observed that the phosphoproteome coverage achievable using TiO2, ZrO2, Fe2O3 and In2O3 is comparable in depth, although some differences between individual oxides exist. Single-shot LC-MS/MS experiments on a linear ion trap-orbitrap instrument identified more than 3000 phosphopeptides from the equivalent of 20 μg of HEK cell lysate protein. However, the application of common enrichment protocols to biological samples may still lead to some bias due to an underrepresentation of highly phosphorylated peptides. These peptides are predominantly observed when materials with lower phosphopeptide affinity such as NiO and Co3O4 are used for enrichment and their biological relevance may be underestimated.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_03:58:55.841.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlexander Leitner
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-07-31 07:28:15ID requested
12017-05-19 01:28:42announced
22024-10-22 03:59:01announced2024-10-22: Updated project metadata.
Publication List
10.1039/c7an00570a;
Leitner A, Sakeye M, Zimmerli CE, Sm, å, tt JH, Insights into chemoselectivity principles in metal oxide affinity chromatography using tailored nanocast metal oxide microspheres and mass spectrometry-based phosphoproteomics. Analyst, 142(11):1993-2003(2017) [pubmed]
Keyword List
curator keyword: Technical
submitter keyword: Phosphoproteomics, human, Orbitrap Elite, phosphopeptide enrichment, HEK293
Contact List
Alexander Leitner
contact affiliationETH Zurich, Institute of Molecular Systems Biology
contact emailleitner@imsb.biol.ethz.ch
lab head
Alexander Leitner
contact affiliationETH Zurich, Institute of Molecular Systems Biology
contact emailleitner@imsb.biol.ethz.ch
dataset submitter
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Dataset FTP location
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PRIDE project URI
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