PXD002643

PXD002643 is an original dataset announced via ProteomeXchange.

Title	Identification of multiple transferrin species in spleen from mice with collagen-induced arthritis which may reflect changes in transferrin glycosylation associated with the disease activity. Role of CD38. Set 2 of identified proteins (MALDI TOF/TOF UltrafleXtreme, Bruker).
Description	Collagen type II-induced arthritis (CIA) is an autoimmune disease that is accompanied by a complex host systemic response, which includes inflammatory and autoimmune reactions. Since to develop CIA is required the injection of antigen in complete Freund’s adjuvant (CFA), which is a water-in-mineral-oil emulsion containing killed Mycobacteria, systemic response proteins related with inflammation can confound the detection or diagnosis of arthritis. CIA in CD38 deficient mice (CD38 KO) is milder than that in C57BL/6 (B6 WT) mice. Protein extracts from spleen were subjected to 2D-DiGE and MS-MALDI-TOF/TOF analysis to identify proteins that were differentially expressed in CD38 KO and B6 WT mice with arthritis, with inflammation, or non-immunized. Differential protein expression was validated by ELISA, or Western-blotting.
HostingRepository	PRIDE
AnnounceDate	2015-12-10
AnnouncementXML	Submission_2015-12-10_03:07:52.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002643
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Antonio Rosal-Vela
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-31 03:20:33	ID requested
⏵ 1	2015-12-10 03:07:53	announced

Rosal-Vela A, Barroso A, Gim, é, nez E, Garc, í, a-Rodr, í, guez S, Longobardo V, Postigo J, Iglesias M, Lario A, Merino J, Merino R, Zubiaur M, Sanz-Nebot V, Sancho J, Identification of multiple transferrin species in the spleen and serum from mice with collagen-induced arthritis which may reflect changes in transferrin glycosylation associated with disease activity: The role of CD38. J Proteomics, 134():127-137(2016) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: CD38, Rheumatoid arthritis, Inflammation, Protein species, Proteoforms, 2D-DIGE

Jaime Sancho Lopez
contact affiliation	Instituto de Parasitología y Biomedicina "López - Neyra", CSIC Departamento de Biología Celular e Inmunología Parque Tecnológico de Ciencias de la Salud, Avda. del Conocimiento, s/n 18016, Granada-Spain
contact email	granada@ipb.csic.es
lab head
Antonio Rosal-Vela
contact affiliation	PostDoc researcher
contact email	arosal@ipb.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002643
     1. Label: PRIDE project
     2. Name: Identification of multiple transferrin species in spleen from mice with collagen-induced arthritis which may reflect changes in transferrin glycosylation associated with the disease activity. Role of CD38. Set 2 of identified proteins (MALDI TOF/TOF UltrafleXtreme, Bruker).