PXD002640

PXD002640 is an original dataset announced via ProteomeXchange.

Title	Protein complex map of Trypanosoma brucei
Description	The functions of the majority of trypanosomatid-specific proteins are unknown, hindering our understanding of the biology and pathogenesis of Trypanosomatida. While protein-protein interactions are highly informative about protein function, a global map of protein interactions and complexes is still lacking for these important human parasites. Here, benefiting from in-depth biochemical fractionation, we systematically examined the protein complexes of more than 3354 protein groups in procyclic life stage of Trypanosoma brucei, the protozoan parasite responsible for human African trypanosomiasis. Using a rigorous methodology, our analysis led to identification of 128 high-confidence complexes encompassing 716 protein groups, including 635 protein groups that had no available experimental annotation. These complexes correlate well with known pathways as well as for proteins co-expressed across the T. brucei life cycle, and provide potential functions for a large number of previously uncharacterized proteins. We validated the functions of several novel proteins associated with the RNA-editing machinery, identifying candidates potentially involved in the life stage specific post-transcriptional regulation in T. brucei. Our data provide an unprecedented view of the protein complex map of T. brucei, and serve as a reliable resource for further characterization of trypanosomatid proteins.
HostingRepository	PRIDE
AnnounceDate	2016-02-29
AnnouncementXML	Submission_2016-03-21_01:38:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Reza Salavati
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion ETD; LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-31 00:44:45	ID requested
1	2016-02-29 08:41:19	announced
⏵ 2	2016-03-21 01:38:47	announced	Updated publication reference for PubMed record(s): 26991453.

Gazestani VH, Nikpour N, Mehta V, Najafabadi HS, Moshiri H, Jardim A, Salavati R, A Protein Complex Map of Trypanosoma brucei. PLoS Negl Trop Dis, 10(3):e0004533(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: trypanosomatids, protein complex map, biochemical fractionation, mass spectrometry

Reza Salavati
contact affiliation	Institute of Parasitology, McGill University, 21111 Lakeshore Road, Ste. Anne de Bellevue, Montreal, Quebec H9X3V9, Canada
contact email	reza.salavati@mcgill.ca
lab head
Reza Salavati
contact affiliation	McGill University
contact email	reza.salavati@mcgill.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD002640

PRIDE project URI

• PRIDE
  1. PXD002640
     1. Label: PRIDE project
     2. Name: Protein complex map of Trypanosoma brucei