PXD002628

PXD002628 is an original dataset announced via ProteomeXchange.

Title	Bodo saltans proteomics
Description	The evolution of parasitism is a recurring event in the history of life and a core question in evolutionary biology. Trypanosomatids are important parasites including the human pathogens Trypanosoma brucei, T. cruzi and Leishmania spp., which have evolved complex life cycles to exploit a series of defined host environments after diverging from free-living, phagotrophic bodonids. However, the origins of genomic adaptations for transmission, disease and pathogenesis remain obscure because there has been no genomic comparison of parasitic and free-living species. Addressing this absence, we have produced a genome sequence for Bodo saltans, the closest known non-parasitic relative of trypanosomatids. Here we show how genomic reduction and innovation contributed to the character of trypanosomatid genomes. We find that despite a genetic ‘streamlining’ of diverse physiological functions, including macromolecular degradation and cellular homeostasis, the origin of trypanosomatid parasitism did not lead to a substantial reduction in genome function. Instead, we observe dramatic elaboration of gene families that facilitate host-parasite interactions and pathogenesis. We also show how parasite-specific proteins that characterize the enigmatic cell surfaces of Trypanosoma and Leishmania were derived from the same ancestral proteins, still represented in B. saltans. Our new evidence distinguishes adaptive innovations of trypanosomatids that post-date their parasitic origin from essentially kinetoplastid legacies of a free-living past. It shows that when the labile environment of a phagotrophic ancestor was replaced by the defined conditions of their various hosts, trypanosomatid physiology was reoriented towards host interaction, and ancestral structures were radically transformed to provide adaptations for obligate parasitism.
HostingRepository	PRIDE
AnnounceDate	2018-10-24
AnnouncementXML	Submission_2018-10-24_08:08:48.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002628
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Stuart Armstrong
SpeciesList	scientific name: Bodo saltans (Flagellated protozoan); NCBI TaxID: 75058;
ModificationList	monohydroxylated residue: 15.994915; iodoacetamide - site C: 57.021464
Instrument	Q Exactive; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-29 07:19:07	ID requested
⏵ 1	2018-10-24 08:08:49	announced

Jackson AP, Otto TD, Aslett M, Armstrong SD, Bringaud F, Schlacht A, Hartley C, Sanders M, Wastling JM, Dacks JB, Acosta-Serrano A, Field MC, Ginger ML, Berriman M, Kinetoplastid Phylogenomics Reveals the Evolutionary Innovations Associated with the Origins of Parasitism. Curr Biol, 26(2):161-172(2016) [pubmed]

curator keyword: Biological

submitter keyword: Bodo saltans, LC-MS/MS

Prof. Julian A. Hiscox
contact affiliation	Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Liverpool, UK.
contact email	julianh@liverpool.ac.uk
lab head
Stuart Armstrong
contact affiliation	Infection Biology
contact email	sarmstro@liv.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD002628

PRIDE project URI

• PRIDE
  1. PXD002628
     1. Label: PRIDE project
     2. Name: Bodo saltans proteomics