PXD002625

PXD002625 is an original dataset announced via ProteomeXchange.

Title	StoS and SreKRS iTRAQ profiling
Description	Xanthomonas oryzae pv. oryzae (Xoo) is a notorious rice pathogen that causes the destructive rice disease bacterial blight. Two-component signal transduction systems (TCSs) are widely used by bacteria to sense and adapt to the environment. However, the profound mechanism how the TCSs execute the environmental adaption remains elusive. In this study, genome-wide TCSs gene knockout in Xoo PXO99A found that StoS (stress tolerance-related oxygen sensor) and SreKRS (salt response kinase, regulator, and sensor) positively regulate EPS production and swarming. Surprisingly, the compromise of two important virulence traits and stress resistance did not attenuate the virulence. To better understand intrinsic function of StoS and SreKRS, quantitative proteomic isobaric tags for relative and absolute quantitation (iTRAQ) was employed. iTRAQ data indicated that carbohydrate metabolisms proteins and chemotaxis proteins, which could be responsible for the EPS and swarming regulation, respectively, were down-regulated in stoS and sreK mutants. Consistent with stoS and sreK mutants exhibiting similar phenotype, the signaling circuits of StoS and SreKRS were found to overlap each other. Moreover, StoS and SreKRS were revealed to moderate expression of the major virulence factor, hypersensitive response and pathogenicity (Hrp) proteins by HrpG-HrpX circuit. Most important, we found that the Xoo strain equipped with StoS and SreKRS outcompete the strain without StoS or SreKRS co-infecting rice and growing outside host. Our findings imply the two TCSs, StoS and SreKRS, could confer environmental fitness on Xoo. Therefore, here we proposed that StoS and SreKRS adopt a novel strategy by moderating Hrp protein expression together with promoting the EPS and motility to adapt to environment.
HostingRepository	PRIDE
AnnounceDate	2016-03-14
AnnouncementXML	Submission_2016-03-14_05:13:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	lifang ruan
SpeciesList	scientific name: Xanthomonas oryzae pv. oryzae (strain PXO99A); NCBI TaxID: 360094;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-29 05:36:02	ID requested
⏵ 1	2016-03-14 05:13:40	announced

Zheng D, Yao X, Duan M, Luo Y, Liu B, Qi P, Sun M, Ruan L, Two overlapping two-component systems in Xanthomonas oryzae pv. oryzae contribute to full fitness in rice by regulating virulence factors expression. Sci Rep, 6():22768(2016) [pubmed]

curator keyword: Biological

submitter keyword: Xanthomonas iTRAQ TCS

Lifang Ruan
contact affiliation	Huazhong Agricultural University
contact email	ruanlifang@mail.hzau.edu.cn
lab head
lifang ruan
contact affiliation	Huazhong Agricultural University
contact email	ruanlifang@mail.hzau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/03/PXD002625

PRIDE project URI

• PRIDE
  1. PXD002625
     1. Label: PRIDE project
     2. Name: StoS and SreKRS iTRAQ profiling