PXD002614

PXD002614 is an original dataset announced via ProteomeXchange.

Title	Quantitative phosphoproteome analysis of glioblastoma stem cells
Description	Glioblastoma is one of the most malignant brain tumors with poor prognosis and their development and progression are known to be driven by glioblastoma stem cells. Although glioblastoma stem cells lose their cancer stemness properties during cultivation in serum-containing medium, little is known about the molecular mechanisms regulating signaling alteration in relation to reduction of stemness. In order to elucidate the global phosphorylation-related signaling events, we performed a SILAC-based quantitative phosphoproteome analysis of serum-induced dynamics in glioblastoma stem cells established from the tumor tissues of the patient. Among a total of 2,876 phosphorylation sites on 1,584 proteins identified in our analysis, 732 phosphorylation sites on 419 proteins were regulated through the alteration of stem cell characteristics.
HostingRepository	PRIDE
AnnounceDate	2016-01-13
AnnouncementXML	Submission_2016-01-13_08:58:11.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002614
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yuta Narushima
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Phospho; Gln->pyro-Glu; Label:13C(6)15N(4); Acetyl; Carbamidomethyl; Label:13C(6)15N(2)
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-28 06:13:25	ID requested
⏵ 1	2016-01-13 08:58:13	announced

Narushima Y, Kozuka-Hata H, Koyama-Nasu R, Tsumoto K, Inoue J, Akiyama T, Oyama M, Integrative Network Analysis Combined with Quantitative Phosphoproteomics Reveals Transforming Growth Factor-beta Receptor type-2 (TGFBR2) as a Novel Regulator of Glioblastoma Stem Cell Properties. Mol Cell Proteomics, 15(3):1017-31(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Quantitative phosphoproteomics, SILAC, nanoLC-MS/MS, Human, Glioblastoma stem cells

Masaaki Oyama
contact affiliation	Medical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo, Japan
contact email	moyama@ims.u-tokyo.ac.jp
lab head
Yuta Narushima
contact affiliation	The Institute of Medical Science, The University of Tokyo
contact email	otherplace9116@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002614

PRIDE project URI

• PRIDE
  1. PXD002614
     1. Label: PRIDE project
     2. Name: Quantitative phosphoproteome analysis of glioblastoma stem cells