PXD002609

PXD002609 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Quantitative Phosphoproteomics reveals new roles for PP6 in chromosome condensation and segregation
Description	Protein phosphorylation by protein kinases and phosphatases is an important regulatory mechanism that controls mitotic progression. Protein Phosphatase 6 (PP6) is an essential enzyme with conserved roles in chromosome segregation and spindle assembly from yeast to humans. Here, we develop a baculovirus-mediated gene silencing approach and combine it with mass spectrometry-based quantitative phosphoproteomics to overcome the lack of PP6-specific inhibitors and comprehensively determine changes in phosphorylation and protein abundance upon depletion of the catalytic subunit of PP6 (PP6c). We identify 400 phosphopeptides on 267 proteins that increase in phosphorylation occupancy upon PP6c depletion in mitosis and reveal new PP6c-dependent regulatory pathways. We demonstrate that PP6c directly opposes casein kinase 2-dependent phosphorylation of the condensin I subunit NCAP-G and show that depletion of PP6c results in defects in chromosome and condensation and segregation in anaphase, consistent with deregulation of condensin I function.
HostingRepository	MassIVE
AnnounceDate	2015-09-22
AnnouncementXML	Submission_2015-09-22_13:33:25.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mark Adamo
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Oxidation; Phospho; Dimethyl; Dimethyl:2H(6)13C(2); TMT6plex
Instrument	Orbitrap Fusion; instrument model: Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-27 12:59:53	ID requested
⏵ 1	2015-09-22 13:33:25	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Mitosis, Protein Phosphatase 6, PP6, Phosphoproteomics

submitter keyword: Mitosis, Protein Phosphatase 6, PP6, Phosphoproteomics

Contact List

Arminja Kettenbach
lab head
Mark Adamo
contact affiliation	Dartmouth College
contact email	mark.e.adamo@dartmouth.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079201