PXD002608

PXD002608 is an original dataset announced via ProteomeXchange.

Title	Proteomic changes in liver of Channa striatus in response to high temperature stress
Description	Climate change and resulting global warming are challenging environmental problems. Understanding the thermal adaptation mechanisms and survival strategies are of paramount importance. Fish has served as excellent animal model for understanding many biological processes. The present study was undertaken to investigate the proteomic changes in liver of murrel Channa striatus exposed to high temperature stress. Fishes were exposed to 36 °C for 4 days and liver proteome changes were analyzed using gel- based proteomics i.e. 2D gel electrophoresis, MALDI-TOF-MS and validation by transcript analysis. The study showed, besides others, up regulation of two sets of proteins, the antioxidant enzymes SOD, ferritin, GST and chaperones HSP60, PDI which was validated by transcript analysis. Further, gene expression analysis was also carried out in the fishes exposed to thermal stress for longer duration (30 days, in the laboratory and beyond, taking Channa collected from a hot spring runoff at a water temperature 36-38 °C); hsp60, sod and gst were found to continue to remain up regulated at 11, 8 and 3 folds, respectively in the hot spring runoff fish. Thus the study showed that SOD, GST and HSP60 play important role in thermal adaptation and survival under chronic heat stress.
HostingRepository	PRIDE
AnnounceDate	2016-04-12
AnnouncementXML	Submission_2016-04-12_00:38:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bimal Mohanty
SpeciesList	scientific name: Channa striata (Snakehead murrel) (Ophicephalus striatus); NCBI TaxID: 64152;
ModificationList	iodoacetamide derivatized residue
Instrument	AB SCIEX instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-27 06:19:17	ID requested
⏵ 1	2016-04-12 00:38:15	announced

Mahanty A, Purohit GK, Banerjee S, Karunakaran D, Mohanty S, Mohanty BP, Proteomic changes in the liver of Channa striatus in response to high temperature stress. Electrophoresis, 37(12):1704-17(2016) [pubmed]

curator keyword: Biological

submitter keyword: Liver, Heat stress, Channa striatus, 2DGE, MALDI-TOF-MS

Bimal Prasanna Mohanty
contact affiliation	ICAR-Central Inland Fisheries Research Institute, Fishery Resourse & Environmental Management Division, Biochemistry Lab-1, Proteomics Unit, Barrackpore, Kolkata, India
contact email	bimalmohanty12@rediffmail.com
lab head
Bimal Mohanty
contact affiliation	ICAR-Central Inland Fisheries Research Institute, Barrackpore
contact email	bimalmohanty12@rediffmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD002608

PRIDE project URI

• PRIDE
  1. PXD002608
     1. Label: PRIDE project
     2. Name: Proteomic changes in liver of Channa striatus in response to high temperature stress