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PXD002607

PXD002607 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSlx5/8 alleviates replication stress by promoting mitotic progression
DescriptionMCM-10 vs. wild type yeast differential analysis using label-free relative quantification and directed mass spectometry using RIPPER. Loss of Mcm10 causes replication stress. We uncovered that S. cerevisiae mcm10-1 mutants rely on the SUMO-targeted E3 ubiquitin ligase complex Slx5/8 and SUMO network for survival. How Slx5/8 suppresses replication stress has remained elusive. We developed a novel intensity-based label-free quantitative mass spectrometry approach and identified SUMO conjugates that were either enriched or depleted in mcm10-1 cells. We reasoned that sumoylated proteins that were decreased in mcm10-1 cells were potential Slx5/8 targets. Such candidates included subunits of the chromosome passenger complex (CPC), Bir1 and Sli15, which we verified to be substrates of the Slx5/8 pathway. CPC is known to positively regulate the spindle assembly checkpoint (SAC), and we show here that the ablation of CPC components and other SAC proteins supports growth of mcm10-1 cells by relieving mitotic arrest. Together, our data provide mechanistic insight into how SUMO and Slx5/8 orchestrate the replication stress response to promote cell survival.
HostingRepositoryPRIDE
AnnounceDate2016-04-05
AnnouncementXMLSubmission_2016-04-05_00:40:57.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSusan Van Riper
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-07-27 03:51:35ID requested
12016-04-05 00:40:58announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: Yeast, LC-MS/MS, Directed Mass Spectrometry
Contact List
Anja-Katrin Bielinsky
contact affiliationBiochemistry, Molecular Biology and Biophysics, University of Minnesota
contact emailbieli003@umn.edu
lab head
Susan Van Riper
contact affiliationUniversity of Minnesota
contact emailvanr0014@umn.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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