PXD002607 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Slx5/8 alleviates replication stress by promoting mitotic progression |
Description | MCM-10 vs. wild type yeast differential analysis using label-free relative quantification and directed mass spectometry using RIPPER. Loss of Mcm10 causes replication stress. We uncovered that S. cerevisiae mcm10-1 mutants rely on the SUMO-targeted E3 ubiquitin ligase complex Slx5/8 and SUMO network for survival. How Slx5/8 suppresses replication stress has remained elusive. We developed a novel intensity-based label-free quantitative mass spectrometry approach and identified SUMO conjugates that were either enriched or depleted in mcm10-1 cells. We reasoned that sumoylated proteins that were decreased in mcm10-1 cells were potential Slx5/8 targets. Such candidates included subunits of the chromosome passenger complex (CPC), Bir1 and Sli15, which we verified to be substrates of the Slx5/8 pathway. CPC is known to positively regulate the spindle assembly checkpoint (SAC), and we show here that the ablation of CPC components and other SAC proteins supports growth of mcm10-1 cells by relieving mitotic arrest. Together, our data provide mechanistic insight into how SUMO and Slx5/8 orchestrate the replication stress response to promote cell survival. |
HostingRepository | PRIDE |
AnnounceDate | 2016-04-05 |
AnnouncementXML | Submission_2016-04-05_00:40:57.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Susan Van Riper |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-07-27 03:51:35 | ID requested | |
⏵ 1 | 2016-04-05 00:40:58 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Yeast, LC-MS/MS, Directed Mass Spectrometry |
Contact List
Anja-Katrin Bielinsky |
contact affiliation | Biochemistry, Molecular Biology and Biophysics, University of Minnesota |
contact email | bieli003@umn.edu |
lab head | |
Susan Van Riper |
contact affiliation | University of Minnesota |
contact email | vanr0014@umn.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD002607 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002607
- Label: PRIDE project
- Name: Slx5/8 alleviates replication stress by promoting mitotic progression