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PXD002605

PXD002605 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTracking the dynamic relationship between cellular systems and extracellular subproteomes in Pseudomonas aeruginosa biofilms
Description. The transition of the opportunistic pathogen Pseudomonas aeruginosa from free-living bacteria into surface-associated biofilm communities represents a viable target for the prevention and treatment of chronic infectious disease. We have established a proteomics platform that identified 2443 and 1142 high-confidence proteins in P. aeruginosa whole cells and outer membrane vesicles (OMVs), respectively, at three time points during biofilm development. Analysis of cellular systems, specifically the phenazine biosynthetic pathway, demonstrates that whole cell protein abundance correlates to end product (i.e., pyocyanin) concentrations in biofilm but not planktonic cultures. Furthermore, increased cellular protein abundance in this pathway results in quantifiable pyocyanin in early biofilm OMVs, and OMVs from both growth modes isolated at later time points. Overall, our data indicate that the OMVs being released from the surface of the biofilm whole cells have unique proteomes in comparison to their planktonic counterparts. The relative abundance of OMV proteins from various subcellular sources showed considerable differences between the two growth modes over time, supporting the existence and preferential activation of multiple OMV biogenesis mechanisms under different conditions. The consistent detection of cytoplasmic proteins in the OMV subproteome suggests that these proteins may contribute a small but functionally relevant component to biofilm OMVs. Direct comparisons of outer membrane protein abundance levels between OMVs and whole cells shows ratios that vary greatly from 1:1, and supports previous studies that advocate specific inclusion, or “packaging”, of proteins into OMVs. The detailed analysis of packaged protein groups indicates biogenesis mechanisms that involve untethered, rather than absent, peptidoglycan-binding proteins. Collectively, individual protein and biological system analyses of biofilm OMVs show that drug-binding cytoplasmic proteins and porins are shuttled from the whole cell into the OMVs, potentially contributing to the antibiotic resistance of P. aeruginosa whole cells within biofilms.
HostingRepositoryPRIDE
AnnounceDate2015-09-21
AnnouncementXMLSubmission_2015-09-21_03:02:43.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJonathan Krieger
SpeciesList scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deaminated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-07-27 02:30:54ID requested
12015-09-21 03:02:44announced
Publication List
Park AJ, Murphy K, Surette MD, Bandoro C, Krieger JR, Taylor P, Khursigara CM, Tracking the Dynamic Relationship between Cellular Systems and Extracellular Subproteomes in Pseudomonas aeruginosa Biofilms. J Proteome Res, 14(11):4524-37(2015) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: biofilms, outer membrane vesicles, quantitative proteomics, Pseudomonas aeruginosa, systems biology
Contact List
Cezar M. Khursigara
contact affiliationDepartment of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada, N1G 2W1; bSPARC BioCentre, The Hospital for Sick Children, Toronto, ON, Canada, M5G 0A4.
contact emailckhursig@uoguelph.ca
lab head
Jonathan Krieger
contact affiliationSickKids/University of Toronto
contact emailJonathan.krieger@sickkids.ca
dataset submitter
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