PXD002601

PXD002601 is an original dataset announced via ProteomeXchange.

Title	HYPE mediated AMPylation profiling in human lysates
Description	We describe here the first example of global chemoproteomic screening and substrate validation for HYPE mediated AMPylation in mammalian cell lysate. Through quantitative mass spectrometry-based proteomics coupled with novel chemoproteomic tools providing MS/MS evidence of AMP modification we identified a total of 27 AMPylated proteins, including the previously validated substrate, ER chaperone BiP (HSPA5), along with novel substrates involved in pathways of gene expression, ATP biosynthesis and maintenance of cytoskeleton. This dataset represents the largest library of AMPylated human proteins reported to date and a foundation for substrate-specific investigations that can ultimately decipher the complex biological networks involved in eukaryotic AMPylation.
HostingRepository	PRIDE
AnnounceDate	2015-12-02
AnnouncementXML	Submission_2015-12-02_03:14:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-27 01:30:46	ID requested
⏵ 1	2015-12-02 03:14:36	announced

Broncel M, Serwa RA, Bunney TD, Katan M, Tate EW, Global Profiling of Huntingtin-associated protein E (HYPE)-Mediated AMPylation through a Chemical Proteomic Approach. Mol Cell Proteomics, 15(2):715-25(2016) [pubmed]

curator keyword: Biological

submitter keyword: AMPylation, HYPE, FIC, chemical proteomics

Edward W. Tate
contact affiliation	Imperial College London Department of Chemistry
contact email	e.tate@imperial.ac.uk
lab head
Remigiusz Serwa
contact affiliation	Imperial College London
contact email	r.serwa@imperial.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/12/PXD002601

PRIDE project URI

• PRIDE
  1. PXD002601
     1. Label: PRIDE project
     2. Name: HYPE mediated AMPylation profiling in human lysates