PXD002592

PXD002592 is an original dataset announced via ProteomeXchange.

Title	Western black widow major ampullate silk gland and silk fiber LC-MSMS, Part 1
Description	Spider silk research has largely focused on spidroins, proteins that are the primary components of spider silk fibers. Although a number of spidroins have been characterized, other types of proteins associated with silk synthesis are virtually unknown. Previous comparison of tissue-specific RNAseq libraries identified 647 predicted genes that were differentially expressed in silk glands of the Western black widow, Latrodectus hesperus. Only ~5% of these transcripts encode spidroins and the remaining predicted genes presumably encode other proteins associated with silk production. Here, we used proteomic analysis of multiple silk glands and dragline silk fiber to investigate the translation of the differentially expressed genes. We find 48 proteins encoded by the differentially expressed transcripts in L. hesperus major ampullate, minor ampullate, and tubuliform silk glands, and detect 16 SST encoded proteins in major ampullate silk fibers. The observed proteins include known silk-related proteins, but most are uncharacterized, with no annotation. These unannotated proteins likely include novel silk associated proteins. Major ampullate and minor ampullate glands have the highest overlap of identified proteins, consistent with their shared, distinctive ampullate shape and the overlapping functions of major ampullate and minor ampullate silks. Our study substantiates and prioritizes predictions from differential expression analysis of spider silk gland transcriptomes.
HostingRepository	PRIDE
AnnounceDate	2015-08-25
AnnouncementXML	Submission_2015-08-25_04:06:25.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002592
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ro Crystal Chaw
SpeciesList	scientific name: Latrodectus hesperus (Western black widow spider); NCBI TaxID: 256737;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-23 00:40:19	ID requested
⏵ 1	2015-08-25 04:06:26	announced

Chaw RC, Correa-Garhwal SM, Clarke TH, Ayoub NA, Hayashi CY, Proteomic Evidence for Components of Spider Silk Synthesis from Black Widow Silk Glands and Fibers. J Proteome Res, 14(10):4223-31(2015) [pubmed]

curator keyword: Biological

submitter keyword: Latrodectus hesperus, major ampullate silk glands, major ampullate silk fibers, draglines, spidroins, spider

Cheryl Y Hayashi
contact affiliation	Department of Biology University of California, Riverside
contact email	cheryl.hayashi@ucr.edu
lab head
Ro Crystal Chaw
contact affiliation	University of California, Riverside
contact email	rcrystal@ucr.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002592

PRIDE project URI

• PRIDE
  1. PXD002592
     1. Label: PRIDE project
     2. Name: Western black widow major ampullate silk gland and silk fiber LC-MSMS, Part 1