PXD002588

PXD002588 is an original dataset announced via ProteomeXchange.

Title	A Conformationally Arrested Peptidomimetic Antibiotic Disrupts Selectively the Outer Membrane in Escherichia coli
Description	Using a shotgun proteomics approach, the expressed proteome of total membrane fractions of Escherichia coli ATCC25922 cells was analyzed under i) normal growth, and ii) treatment with the novel peptidomimetic JB-95 (a structurally arrested cyclic peptide). By selectively disrupturing the outer membrane (OM), this peptidomimetic displayed a novel mechanism of action and opens new avenues for developing antibiotics that specifically target the OM of Gram-negative bacteria.
HostingRepository	PRIDE
AnnounceDate	2018-04-26
AnnouncementXML	Submission_2018-04-26_05:31:28.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002588
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christian Ahrens
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	Oxidation; unknown modification
Instrument	LTQ Orbitrap Velos; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-22 02:53:13	ID requested
1	2015-12-07 10:08:58	announced
⏵ 2	2018-04-26 05:31:29	announced	Updated project metadata.

Urfer M, Bogdanovic J, Lo Monte F, Moehle K, Zerbe K, Omasits U, Ahrens CH, Pessi G, Eberl L, Robinson JA, A Peptidomimetic Antibiotic Targets Outer Membrane Proteins and Disrupts Selectively the Outer Membrane in Escherichia coli. J Biol Chem, 291(4):1921-1932(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: E. coli, novel antibiotic, arrested cyclic peptide, OM rupture, shotgun proteomics, total membrane fraction, new MoA

Prof. John Robinson
contact affiliation	Chemistry Department, University of Zurich, Zurich, Switzerland
contact email	John.Robinson@chem.uzh.ch
lab head
Christian Ahrens
contact affiliation	Agroscope
contact email	christian.ahrens@agroscope.admin.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/12/PXD002588

PRIDE project URI

• PRIDE
  1. PXD002588
     1. Label: PRIDE project
     2. Name: A Conformationally Arrested Peptidomimetic Antibiotic Disrupts Selectively the Outer Membrane in Escherichia coli