PXD002582

PXD002582 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis reveals distinct metabolic differences between GM-CSF and M-CSF grown macrophages derived from murine bone marrow cells
Description	Macrophages are crucial in controlling infectious agents and tissue homeostasis. Macrophages require a wide range of functional capabilities in order to fulfill distinct roles in our body, one being rapid and robust immune responses. To gain insight into macrophage plasticity and the key regulatory protein networks governing their specific functions, we performed quantitative analyses of the proteome and phosphoproteome of murine primary GM-CSF and M-CSF grown bone marrow derived macrophages (GM-BMMs and M-BMMs, respectively) using the latest isobaric tag based tandem mass tag (TMT) labeling and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Strikingly, metabolic processes emerged as a major difference between these macrophages. Specifically, GM-BMMs show significant enrichment of proteins involving glycolysis, the mevalonate pathway, and nitrogen compound biosynthesis. This evidence of enhanced glycolytic capability in GM-BMMs is particularly significant regarding their pro-inflammatory responses, because increased production of cytokines upon LPS stimulation in GM-BMMs depends on their acute glycolytic capacity. In contrast, M-BMMs upregulate proteins involved in endocytosis, which correlates with a tendency toward homeostatic functions such as scavenging cellular debris. Together, our data describes a proteomic network that underlies the pro-inflammatory actions of GM-BMMs as well as the homeostatic functions of M-BMMs.
HostingRepository	PRIDE
AnnounceDate	2015-08-04
AnnouncementXML	Submission_2015-08-04_11:05:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ji Hye Hong
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-21 05:41:10	ID requested
⏵ 1	2015-08-04 11:05:39	announced

Na YR, Hong JH, Lee MY, Jung JH, Jung D, Kim YW, Son D, Choi M, Kim KP, Seok SH, Proteomic Analysis Reveals Distinct Metabolic Differences Between Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) and Macrophage Colony Stimulating Factor (M-CSF) Grown Macrophages Derived from Murine Bone Marrow Cells. Mol Cell Proteomics, 14(10):2722-32(2015) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Mass spectrometry, Phosphoproteins, Protein Identification, Macrophage, TMT, Immunology

Kwang Pyo Kim
contact affiliation	Department of Applied Chemistry College of Applied Sciences, Kyung Hee University Yong-in City, 446-701 Republic of Korea
contact email	kimkp@khu.ac.kr
lab head
Ji Hye Hong
contact affiliation	South Korea
contact email	jhhong829@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002582
     1. Label: PRIDE project
     2. Name: Proteomic analysis reveals distinct metabolic differences between GM-CSF and M-CSF grown macrophages derived from murine bone marrow cells