PXD002581

PXD002581 is an original dataset announced via ProteomeXchange.

Title	Peanut LC-MS/MS, part 3 of 3
Description	Cultivated peanut (Arachis hypogaea. L) is one of the most important oil crops in the world. After flowering, the peanut plant forms aboveground pegs (gynophores) that penetrate the soil, giving rise to underground pods. This means of reproduction, referred to as geocarpy, distinguishes peanuts from most other plants. The molecular mechanism underlying geocarpic pod development in peanut is poorly understood. To gain insight into the mechanism of geocarpy, we extracted proteins from aerial gynophores, subterranean unswollen gynophores, and gynophores that had just started to swell into pods. We analyzed the protein profiles in each of these samples by combining 1 DE with nanoLC-MS/MS approaches. In total, 2766, 2518, and 2280 proteins were identified from the three samples, respectively. An integrated analysis of proteome and transcriptome data revealed specifically or differentially expressed genes in the different developmental stages at both the mRNA and protein levels. A total of 69 proteins involved in the gravity response, light and mechanical stimulus, hormone biosynthesis, and transport were identified as being involved in geocarpy. Furthermore, we identified 91 genes that were specifically or abundantly expressed in aerial gynophores, including pectin methylesterase and expansin, which were presumed to promote the elongation of aerial gynophores. In addition, we identified 35 proteins involved in metabolism, defense, hormone biosynthesis and signal transduction, nitrogen fixation, and transport that accumulated in subterranean unswellen gynophores. Furthermore, 26 specific or highly abundant proteins related to fatty acid metabolism, starch synthesis, and lignin synthesis were identified in the early swelling pods. We identified thousands of proteins in the aerial gynophores, subterranean gynophores, and early swelling pods of peanut. This study provides the basis for examining the molecular mechanisms underlying peanut geocarpy pod development.
HostingRepository	PRIDE
AnnounceDate	2015-08-11
AnnouncementXML	Submission_2015-08-10_16:58:34.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002581
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chuanzhi Zhao
SpeciesList	scientific name: Arachis hypogaea (Peanut); NCBI TaxID: 3818;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deaminated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-21 05:07:47	ID requested
⏵ 1	2015-08-10 16:58:35	announced

Zhao C, Zhao S, Hou L, Xia H, Wang J, Li C, Li A, Li T, Zhang X, Wang X, Proteomics analysis reveals differentially activated pathways that operate in peanut gynophores at different developmental stages. BMC Plant Biol, 15():188(2015) [pubmed]

curator keyword: Biological

submitter keyword: Peanut, gynophores, pods

Xingjun Wang
contact affiliation	Bio-tech Research center, Shandong Academy of Agricultural Science, Jinan, China.
contact email	xingjunw@hotmail.com
lab head
Chuanzhi Zhao
contact affiliation	Bio-Tech Research Center, Shandong Academy of Agricultural Sciences
contact email	chuanzhiz@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002581
     1. Label: PRIDE project
     2. Name: Peanut LC-MS/MS, part 3 of 3