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PXD002575

PXD002575 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleZea mays AM fungi interaction
DescriptionWe describe a method that detects membrane proteins from cell type-specific plant plasma membrane subdomains. As the biochemical properties of membrane subdomains are often unknown, general membrane enrichment by continuous sucrose density gradient centrifugation was combined with filter-aided sample preparation, strong cation exchange pre-fractionation and sensitive LTQ Orbitrap Velos-based mass-spectrometry proteomics. This workflow identified plasma membrane proteins, including underrepresented proteins, at an unprecedented level of sensitivity and reproducibility.
HostingRepositoryPRIDE
AnnounceDate2018-09-24
AnnouncementXMLSubmission_2018-11-13_06:00:43.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD002575
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMarco Chiapello
SpeciesList scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationListGlu->pyro-Glu; Oxidation; Carbamidomethyl; Gln->pyro-Glu
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-07-21 02:09:33ID requested
12018-09-24 06:41:15announced
22018-11-13 06:00:44announcedUpdated publication reference for PubMed record(s): 30410018.
Publication List
Roth R, Chiapello M, Montero H, Gehrig P, Grossmann J, O'Holleran K, Hartken D, Walters F, Yang SY, Hillmer S, Schumacher K, Bowden S, Craze M, Wallington EJ, Miyao A, Sawers R, Martinoia E, Paszkowski U, A rice Serine/Threonine receptor-like kinase regulates arbuscular mycorrhizal symbiosis at the peri-arbuscular membrane. Nat Commun, 9(1):4677(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: proteomics AM fungi Maize plasma membrabe
Contact List
Uta Paszkowski
contact affiliationUniversity of Cambridge
contact emailup220@cam.ac.uk
lab head
Marco Chiapello
contact affiliationUniversità degli Studi di Torino
contact emailchiapello.m@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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