PXD002573

PXD002573 is an original dataset announced via ProteomeXchange.

Title	Translational regulation via ribosome modification
Description	This dataset compares the proteomes of WT P. fluorescens and a delta-hfq mutant strain. This forms part of our characterisation of a new mechanism for translational control in bacteria, based on the covalent modification of a ribosomal protein by the enzyme RimK. RimK modification induces specific effects on ribosomal stability and function, which in turn affects the abundance of the important translational regulator Hfq. RimK is itself controlled by binding to the small regulatory proteins RimA and RimB and the widespread signalling molecule cyclic-di-GMP. Deletion of rimK compromises motility, virulence and plant colonisation/infection in several different Pseudomonas species. Changes in intracellular RimK activity enable Pseudomonas to translate environmental pressures into dynamic ribosomal changes, and in turn into adaptive phenotypic responses to the surroundings. This promotes motility and virulence during the initial stages of plant contact, and phenotypes including attachment, metabolite transport and stress control during long-term environmental adaptation.
HostingRepository	PRIDE
AnnounceDate	2016-02-17
AnnouncementXML	Submission_2016-02-17_06:50:31.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002573
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gerhard Saalbach
SpeciesList	scientific name: Pseudomonas fluorescens (strain SBW25); NCBI TaxID: 216595;
ModificationList	Oxidation; Acetyl; Carbamidomethyl; Deamidated
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-21 02:04:20	ID requested
⏵ 1	2016-02-17 06:50:32	announced

Little RH, Grenga L, Saalbach G, Howat AM, Pfeilmeier S, Trampari E, Malone JG, Adaptive Remodeling of the Bacterial Proteome by Specific Ribosomal Modification Regulates Pseudomonas Infection and Niche Colonisation. PLoS Genet, 12(2):e1005837(2016) [pubmed]

curator keyword: Biological

submitter keyword: Ribosomal modification, translational control, Pseudomonas, LC-MS/MS

Dr Jacob George Malone
contact affiliation	University of East Anglia/John Innes Centre
contact email	jacob.malone@jic.ac.uk
lab head
Gerhard Saalbach
contact affiliation	Biological Chemistry
contact email	gerhard.saalbach@jic.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002573
     1. Label: PRIDE project
     2. Name: Translational regulation via ribosome modification