PXD002566

PXD002566 is an original dataset announced via ProteomeXchange.

Title	Characterization and quantification of proteins secreted by single human embryos prior to implantation
Description	The use of in vitro fertilization (IVF) has revolutionized the treatment of infertility and is now responsible for 1-5% of all births in industrialized countries. During IVF, it is typical for patients to generate multiple embryos. However, only a small proportion of them possess the genetic and metabolic requirements needed in order to produce a healthy pregnancy. The identification of the embryo with the greatest developmental capacity represents a major challenge for fertility clinics. Current methods for the assessment of embryo competence are proven inefficient and the inadvertent transfer of non-viable embryos is the principal reason why most IVF treatments (approximately two-thirds) end in failure. In this study, we investigate how the application of proteomic measurements could improve success rates in clinical embryology. We describe a procedure that allows the identification and quantification of proteins of embryonic origin, present in attomole concentrations in the blastocoel, the enclosed fluid filled cavity that forms within five-day old human embryos. By using targeted proteomics, we demonstrate the feasibility of quantifying multiple proteins in samples derived from single blastocoels, and that such measurements correlate with aspects of embryo viability, such as chromosomal (ploidy) status. This study illustrates the potential of high-sensitivity proteomics to measure clinically relevant biomarkers in minute samples and, more specifically, suggests that key aspects of embryo competence could be measured using a proteomic-based strategy, with negligible risk of harm to the living embryo. Our work paves the way for the development of “next-generation” embryo competence assessment strategies, based on functional proteomics.
HostingRepository	PRIDE
AnnounceDate	2015-10-20
AnnouncementXML	Submission_2015-10-20_03:48:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alessandro Ori
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-20 08:04:23	ID requested
⏵ 1	2015-10-20 03:48:21	announced

Poli M, Ori A, Child T, Jaroudi S, Spath K, Beck M, Wells D, Characterization and quantification of proteins secreted by single human embryos prior to implantation. EMBO Mol Med, 7(11):1465-79(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: IVF, secretion, human blastocyst, blastocoel

Dagan Wells
contact affiliation	Nuffield Department of Obstetrics and Gynaecology, University of Oxford, Institute of Reproductive Sciences, Oxford, OX4 2HW, UK
contact email	dagan.wells@obs-gyn.ox.ac.uk
lab head
Alessandro Ori
contact affiliation	EMBL
contact email	aleori@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002566
     1. Label: PRIDE project
     2. Name: Characterization and quantification of proteins secreted by single human embryos prior to implantation