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PXD002561

PXD002561 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of cell cycle dependent interaction partners of the septins by quantitative mass spectrometry
DescriptionThe septins are a conserved family of GTP-binding proteins that, in the baker's yeast, assemble into a highly ordered array of filaments at the mother bud neck. These filaments undergo dramatic structural rearrangements during the cell cycle which are mechanistically not yet understood. We aimed at identifying key components that are involved in the transitions of the septins. Combining cell synchronization and quantitative affinity-purification mass-spectrometry (AP-MS), we identified interaction partners of the septins at three distinct stages of the cell cycle. A total of 83 interaction partners of the septins were assigned. We detected DNA-interacting/nuclear proteins and proteins involved in ribosome biogenesis and protein synthesis predominantly present in G1 arrested cells without an assembled septin structure. In addition, two distinct sets of regulatory proteins that are specific for cells at S-phase with a stable septin collar or at mitosis with split septin rings were identified. Complementary methods such as the SPLIFF method allowed us to more exactly define the spatial and temporal characteristics of selected candidate proteins of the AP-MS screen.
HostingRepositoryPRIDE
AnnounceDate2016-01-28
AnnouncementXMLSubmission_2016-02-15_00:18:07.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFriedel Drepper
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-07-20 01:54:09ID requested
12016-01-28 08:01:34announced
22016-02-15 00:18:09announcedUpdated publication reference for PubMed record(s): 26871441.
Publication List
Renz C, Oeljeklaus S, Grinhagens S, Warscheid B, Johnsson N, Gronemeyer T, Identification of Cell Cycle Dependent Interaction Partners of the Septins by Quantitative Mass Spectrometry. PLoS One, 11(2):e0148340(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: AP-MS, cell cycle, quantitative proteomics, septins, SILAC, SPLIFF, yeast
Contact List
Thomas Gronemeyer
contact affiliationUlm University, Department of Molecular Genetics and Cell Biology, Ulm, Germany
contact emailthomas.gronemeyer@uni-ulm.de
lab head
Friedel Drepper
contact affiliationAG Warscheid Biologie II Albert-Ludwigs-Universität Freiburg Schänzlestr. 1 79104 Freiburg Germany
contact emailfriedel.drepper@biologie.uni-freiburg.de
dataset submitter
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Dataset FTP location
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